Abstract：
A high protein retention polyethersulfone (PES) membrane was prepared by nonsolvent-induced phase separation and surface coating, which exhibited enhanced hemocompatibility and antioxidant stress performance. The cross-linked network was constructed by tannic acid (TA) and alpha-lipoic acid (α-LA) on the surface of the membrane, which controlled the pores to a reasonable size. The enrichment of heparin-like groups on the membrane surface, implemented by \"hydrophobic interaction\" and \"click reaction\", confers anticoagulant properties; the presence of a large number of phenolic hydroxyl groups from TA and the introduction of α-LA allows the modified membranes to intervene in oxidative stress. The hemocompatibility characterizations included plasma recalcification time (PRT), activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and hemolysis rate (HR). Additionally, the DPPH ABTS radical scavenging capacity was tested to evaluate the antioxidant performance. The results show that the modified membrane presents an outstanding protein retention rate (99.3%) along with permeability. In addition, the PRT is prolonged to 341.7 s, and the DPPH• scavenging ability reaches 0.74 µmol•cm-2. The membranes can be easily prepared and present excellent comprehensive performance. This work provides a simple and facile strategy for the fabrication of hemodialysis membranes with controllable pore sizes.
摘要：
通过非溶剂诱导的相分离和表面涂层制备了高蛋白质保留聚醚砜（PES）膜，表现出增强的血液相容性和抗氧化应激性能。通过单宁酸（TA）和α-硫辛酸（α-LA）在膜表面构建交联网络，将毛孔控制在合理的尺寸。肝素样基团在膜表面的富集，通过“疏水相互作用”和“点击反应”实现，赋予抗凝血性质;来自TA的大量酚羟基的存在和α-LA的引入允许改性膜干预氧化应激。血液相容性特征包括血浆复钙时间（PRT），活化部分凝血活酶时间（APTT），凝血酶原时间（PT），凝血酶时间(TT)和溶血率(HR)。此外,测试了DPPHABTS自由基清除能力以评估其抗氧化性能。结果表明，改性膜具有出色的蛋白质保留率（99.3％）以及渗透性。此外,PRT延长至341.7s，DPPH·清除能力达到0.74µmol·cm-2。该膜易于制备并具有优异的综合性能。这项工作为制造具有可控孔径的血液透析膜提供了简单而简便的策略。
