Abstract：
Sepsis caused by methicillin-resistant coagulase-negative staphylococci (MRCoNS) seriously affects the morbidity and mortality of neonates. However, the hand carriage and genotypic diversity of MRCoNS within surgical staff remain unclear in China. In the study, antimicrobial susceptibility tests and genotypic characterisation were applied to MRCoNS. One hundred and one samples were collected from the hands of gynaecological surgical staff. Eighty staphylococcal isolates were identified, of which 75 (94%) were resistant to at least one antibiotic. mecA gene was determined in 50 (62.5%) staphylococcal isolates. Panton-Valentine leukocidin (pvl) and ica genes were determined in 17 (21%) and 12 (15%) staphylococcal isolates, respectively. About 52% of staphylococci carried SCCmec IV and V, followed by SCCmec type I, II, and III (38%). In addition, two new STs types were assigned as ST844 and ST845. The high level of hand MRCoNS colonisation rate in gynaecological surgical staff is of concern, and hand hygiene management should be emphasised among surgical assistants.Impact statementWhat is already known on this subject? Coagulase-negative staphylococci (CoNS) are the predominant cause of neonatal sepsis. Exposure to antimicrobial-resistant CoNS might put neonates at increased risk of infection. However, little is known about the carriage and genetic diversity of methicillin-resistant CoNS (MRCoNS) of gynaecological surgeons and surgical assistants.What do the results of this study add? This is the first study on the molecular characterisation of MRCoNS recovered from gynaecological surgeons and surgical assistants in China. MRCoNS carriage rate in surgical assistants was significantly higher than in surgeons. Seventy-five (94%) coagulase-negative staphylococci were resistant to at least one antibiotic. SCCmec I, II and III was the dominant types identified in MRCoNS that were recovered from surgical staff. Fifty (62.5%) staphylococcal isolates that were recovered from surgical staff harboured the mecA gene. Pathogenic clones of MRCoNS were disseminated in surgical staff, and half of mecA-positive Staphylococcus epidermidis harboured the ica gene.What are the implications of these findings for clinical practice and/or further research? The high level of hand MRCoNS colonisation rate among gynaecological surgical staff is of concern. The alarming outcome of this study suggested that hygiene measures should be emphasised among gynaecological surgical assistants.
摘要：
耐甲氧西林凝固酶阴性葡萄球菌（MSCoNS）引起的脓毒症严重影响新生儿的发病率和死亡率。然而,在中国，外科工作人员中MRCoNS的手提和基因型多样性尚不清楚。在研究中,将抗菌药物敏感性试验和基因型表征应用于MRCoNS。从妇科手术人员的手中收集了一百一十个样本。鉴定出80株葡萄球菌分离株，其中75（94％）对至少一种抗生素具有抗性。在50株(62.5%)葡萄球菌分离株中检测到mecA基因。在17（21％）和12（15％）葡萄球菌分离株中确定了Panton-Valentine杀白细胞素（pvl）和ica基因，分别。大约52％的葡萄球菌携带SCCmecIV和V，其次是SCCmecI型，II,和III（38％）。此外,两种新的STs类型被分配为ST844和ST845。妇科手术人员的手部MRCoNS定植率高令人担忧，外科助理应重视手卫生管理。凝血酶阴性葡萄球菌（CoNS）是新生儿败血症的主要原因。暴露于耐药性CoNS可能会使新生儿感染风险增加。然而,对妇科外科医生和外科助理的耐甲氧西林CoNS（MSCoNS）的携带和遗传多样性知之甚少。这项研究的结果补充了什么？这是对从中国妇科外科医生和外科助理那里获得的MRCoNS分子表征的第一项研究。手术助手的MRCoNS携带率明显高于外科医生。75例（94％）凝固酶阴性葡萄球菌对至少一种抗生素具有抗性。SCCmecI,II和III是从手术人员中恢复的MRCoNS中确定的主要类型。从外科工作人员中回收的50株（62.5％）葡萄球菌分离株具有mecA基因。MRCoNS的致病克隆在外科工作人员中传播，一半的mecA阳性表皮葡萄球菌携带ica基因。这些发现对临床实践和/或进一步研究有什么意义？妇科手术人员中高水平的手部MRCoNS定植率令人担忧。这项研究的令人震惊的结果表明，妇科手术助手应强调卫生措施。
