PDF(Pubmed)
Abstract：
Bovine respiratory disease (BRD), one of the most common and infectious diseases in the beef industry, is associated with the respiratory microbiome and stressors of transportation. The impacts of the bovine respiratory microbiota on health and disease across different geographic locations and sampling niches are poorly understood, resulting in difficult identification of BRD causes. In this study, we explored the effects of geography and niches on the bovine respiratory microbiome and its function by re-analyzing published metagenomic datasets and estimated the main opportunistic pathogens that changed after transportation. The results showed that diversity, composition, structure, and function of the bovine nasopharyngeal microbiota were different across three worldwide geographic locations. The lung microbiota also showed distinct microbial composition and function compared with nasopharyngeal communities from different locations. Although different signature microbiota for each geographic location were identified, a module with co-occurrence of Mycoplasma species was observed in all bovine respiratory communities regardless of geography. Moreover, transportation, especially long-distance shipping, could increase the relative abundance of BRD-associated pathogens. Lung microbiota from BRD calves shaped clusters dominated with different pathogens. In summary, geography, sampling niches, and transportation are important factors impacting the bovine respiratory microbiome and disease, and clusters of lung microbiota by different bacterial species may explain BRD pathogenesis, suggesting the importance of a deeper understanding of bovine respiratory microbiota in health.
摘要：
牛呼吸道疾病（BRD），牛肉行业中最常见的传染病之一，与呼吸道微生物组和运输压力源有关。牛呼吸道微生物群对不同地理位置和采样生态位的健康和疾病的影响知之甚少。导致难以识别BRD的原因。在这项研究中,我们通过重新分析已发表的宏基因组数据集，探索了地理和生态位对牛呼吸道微生物组及其功能的影响，并估计了运输后变化的主要机会性病原体。结果表明,composition,结构,牛鼻咽部微生物群的功能在全球三个地理位置上有所不同。与来自不同位置的鼻咽群落相比，肺微生物群也显示出不同的微生物组成和功能。虽然每个地理位置的不同特征微生物群被确定，在所有牛呼吸群落中，无论地理位置如何，都观察到一个与支原体物种共存的模块。此外，交通运输,特别是长途运输，可以增加BRD相关病原体的相对丰度。来自BRD小牛形状簇的肺微生物群以不同的病原体为主。总之,地理,采样生态位，和运输是影响牛呼吸道微生物组和疾病的重要因素，不同细菌种类的肺微生物群可以解释BRD的发病机理，表明更深入了解牛呼吸道微生物群对健康的重要性。
