Abstract：
Terbium citrate staining represents the method of choice for RNA visualization at transmission electron microscopy. Because of its affinity for guanosines in RNA rather than in DNA, terbium citrate gives precise results being a selective staining. However, difficulties often arise when performing this technique, especially in crucial and delicate steps such as rinses, when it is not uncommon to excessively reduce the already feeble contrast. For these reasons, we developed a straightforward and secure approach to overcome such complications. Here we report a new method for RNA single molecule localization by means of terbium citrate vapors, viable for every type of fixation and embedding.
摘要：
柠檬酸Tr染色代表了在透射电子显微镜下RNA可视化的选择方法。由于它对RNA而不是DNA中的鸟嘌呤的亲和力，柠檬酸tri给出精确的结果是选择性染色。然而,执行此技术时经常出现困难，尤其是在冲洗等关键和微妙的步骤中，过度减少已经微弱的对比度并不少见。由于这些原因,我们开发了一种简单而安全的方法来克服此类并发症.在这里，我们报告了一种新的RNA单分子定位方法，适用于每种类型的固定和嵌入。
