Abstract：
Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.
摘要：
细胞极性和胆小管的形成可以在由患者来源的诱导多能干细胞产生的肝细胞中实现。这允许研究内源性突变蛋白，患者特异性发病机制，以及对肝细胞极性和胆小管起关键作用的疾病的药物反应。这里,我们描述了从诱导多能干细胞产生胆小管形成肝细胞的分步方案及其评估。
