%0 Journal Article
%T Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells.
%A Matakovic L
%A Overeem AW
%A Klappe K
%A van IJzendoorn SCD
%A Matakovic L
%A Overeem AW
%A Klappe K
%A van IJzendoorn SCD
%A Matakovic L
%A Overeem AW
%A Klappe K
%A van IJzendoorn SCD
%J Methods Mol Biol
%V 2544
%N 0
%D 2022
%M 36125710
暂无%R 10.1007/978-1-0716-2557-6_4
%X Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.