{Reference Type}: Journal Article
{Title}: Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells.
{Author}: Matakovic L;Overeem AW;Klappe K;van IJzendoorn SCD;Matakovic L;Overeem AW;Klappe K;van IJzendoorn SCD;Matakovic L;Overeem AW;Klappe K;van IJzendoorn SCD;
{Journal}: Methods Mol Biol
{Volume}: 2544
{Issue}: 0
{Year}: 2022
暂无{DOI}: 10.1007/978-1-0716-2557-6_4
{Abstract}: Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are generated from patient-derived induced pluripotent stem cells. This allows for the study of endogenous mutant proteins, patient-specific pathogenesis, and drug responses for diseases where hepatocyte polarity and bile canaliculi play a key role. Here, we describe a step-by-step protocol for the generation of bile canaliculi-forming hepatocytes from induced pluripotent stem cells and their evaluation.