Abstract：
OBJECTIVE: Small mammals are important reservoirs of ticks and their pathogens in nature. However, studies reporting these associations are still rare in Brazil. In the present study, we investigated the presence of Rickettsia DNA in ticks parasitizing rodents and marsupials captured in different areas throughout the Atlantic rainforest biome, Bahia, Northeastern (NE), Brazil.
METHODS: The study was conducted in five municipalities within of the Atlantic Forest biome, Bahia state, in NE Brazil. Two campaigns were done in each municipality. For host captures Sherman and Tomahawk traps were used, and pitfall traps. After being captured, the hosts were anesthetized and their entire body examined for ticks. When ticks were detected, they were manually removed and stored in eppendorf tubes (1.5 ml) containing absolute PA ethanol for future laboratory analysis (identification of ticks and detection of Rickettsia spp.).
RESULTS: A total of 609 mammals were captured. Overall, 208 ticks of the genus Amblyomma and Ixodes were collected: A. ovale, I. loricatus and A. varium. Rickettsia DNA was detected in A. ovale and it was 99-100% of identity to the sequence deposited in GenBank as Rickettsia parkeri strain Atlantica rainforest.
CONCLUSIONS: These results suggest that R. parkeri strain Atlantic rainforest occurs in the region, and A. ovale is likely the vector.
METHODS: The study was conducted in five municipalities within of the Atlantic Forest biome, Bahia state, in NE Brazil. Two campaigns were done in each municipality. For host captures Sherman and Tomahawk traps were used, and pitfall traps. After being captured, the hosts were anesthetized and their entire body examined for ticks. When ticks were detected, they were manually removed and stored in eppendorf tubes (1.5 ml) containing absolute PA ethanol for future laboratory analysis (identification of ticks and detection of Rickettsia spp.).
RESULTS: A total of 609 mammals were captured. Overall, 208 ticks of the genus Amblyomma and Ixodes were collected: A. ovale, I. loricatus and A. varium. Rickettsia DNA was detected in A. ovale and it was 99-100% of identity to the sequence deposited in GenBank as Rickettsia parkeri strain Atlantica rainforest.
CONCLUSIONS: These results suggest that R. parkeri strain Atlantic rainforest occurs in the region, and A. ovale is likely the vector.
摘要：
目的：小型哺乳动物是自然界中壁虱及其病原体的重要宿主。然而,报道这些关联的研究在巴西仍然很少见。在本研究中,我们调查了在整个大西洋雨林生物群落不同地区捕获的寄生啮齿动物和有袋动物的蜱虫中立克次体DNA的存在，巴伊亚,东北（NE），巴西。
方法：这项研究是在大西洋森林生物群系内的五个城市进行的，巴伊亚州,在巴西东北部。每个城市都进行了两次运动。对于主机捕获，使用了谢尔曼和战斧陷阱，和陷阱陷阱。被捕获后,宿主被麻醉，他们的整个身体检查蜱。当检测到蜱时，将它们手动取出并储存在含有绝对PA乙醇的eppendorf管(1.5ml)中,用于未来的实验室分析(蜱的鉴定和立克次体的检测.).
结果：共捕获到609只哺乳动物。总的来说,收集了Ablyomma属和Ixodes属的208个蜱虫：卵卵圆，I.loricatus和A.varium。在卵卵圆中检测到立克次体DNA，它与作为立克次体Parkeri菌株Atlantica雨林存放在GenBank中的序列具有99-100％的同一性。
结论：这些结果表明，该地区出现了R.parkeri菌株大西洋雨林，卵圆瓶可能是矢量。
方法：这项研究是在大西洋森林生物群系内的五个城市进行的，巴伊亚州,在巴西东北部。每个城市都进行了两次运动。对于主机捕获，使用了谢尔曼和战斧陷阱，和陷阱陷阱。被捕获后,宿主被麻醉，他们的整个身体检查蜱。当检测到蜱时，将它们手动取出并储存在含有绝对PA乙醇的eppendorf管(1.5ml)中,用于未来的实验室分析(蜱的鉴定和立克次体的检测.).
结果：共捕获到609只哺乳动物。总的来说,收集了Ablyomma属和Ixodes属的208个蜱虫：卵卵圆，I.loricatus和A.varium。在卵卵圆中检测到立克次体DNA，它与作为立克次体Parkeri菌株Atlantica雨林存放在GenBank中的序列具有99-100％的同一性。
结论：这些结果表明，该地区出现了R.parkeri菌株大西洋雨林，卵圆瓶可能是矢量。
