PDF(Pubmed)
Abstract：
Food-borne diseases caused by microbial contamination have always been a matter of great concern to human beings. Hence, the research on these problems has never stopped. With the development of microorganism amplification technology, more and more detection methods have come into our vision. However, traditional detection technologies presents more or less drawbacks, such as complicated operation, low accuracy, low sensitivity, long-time detection, and so on. Therefore, more convenient, accurate, and sensitive measurement for the microorganism are needed. Isothermal amplification technology is one of the alternative approach containing the above mentioned advantages. This work mainly summarizes the principles of loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA) which belong to isothermal amplification. Meanwhile, the application of LAMP and RCA in food microorganism detection is introduced.
摘要：
微生物沾染惹起的食源性疾病一向是人类极其存眷的事宜。因此，对这些问题的研究从未停止过。随着微生物扩增技术的发展,越来越多的检测方法进入了我们的视野。然而,传统的检测技术或多或少存在缺陷，例如复杂的操作，精度低,灵敏度低,长时间检测，等等。因此,更方便,准确,并且需要对微生物进行灵敏的测量。等温扩增技术是包含上述优点的替代方法之一。本文主要总结了环介导等温扩增(LAMP)和滚环扩增(RCA)的原理。同时,介绍了LAMP和RCA在食品微生物检测中的应用。
