Abstract：
The main bioactive constituents in the standardized Ginkgo biloba leaf extract (EGb 761) are the terpene lactones and flavonoid glycosides. EGb 761\'s antioxidant and anti-inflammatory properties have previously been demonstrated. Indomethacin-induced gastric ulcers have a multifactorial etiology and represent a major restriction to its therapeutic utility. The underlying ulcerogenic process involves oxidative and inflammatory biomolecular insults. This study was performed to explore the curative and preventative benefits of EGb 761 in experimentally-induced ulcers. To develop gastric ulcers in mice, indomethacin (40 mg/kg) was administered orally. EGb 761 (200 mg/kg) was given by gavage for 7 days before (preventative) and after (therapeutic) indomethacin administration. The histological alterations and macroscopic mucosal lesions were assessed. In gastric tissue homogenates, malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), and inflammatory cytokines were measured. The expressions of cyclooxygenase-2 (COX-2), cytokines, and proliferating cell nuclear antigen (PCNA) in the stomach mucosa were also investigated. The ulcer index, histological alterations, gastric oxidants, and inflammatory biomarkers were all significantly increased by indomethacin. In stomach specimens, it increased COX-2 and PCNA expression. EGb 761 treatments, both prophylactic and therapeutic, resulted in significant reductions in ulcer lesions, nitrosative and oxidative damage, and inflammatory markers, along with the lowering of COX-2 and PCNA expressions. Furthermore, in the fight against stomach ulcers, EGb 761 treatment was found to be more efficient than prevention.
摘要：
标准银杏叶提取物（EGb761）中的主要生物活性成分是萜内酯和类黄酮苷。EGb761的抗氧化和抗炎特性先前已被证明。吲哚美辛诱导的胃溃疡具有多因素病因，并代表其治疗效用的主要限制。潜在的溃疡形成过程涉及氧化和炎性生物分子损伤。进行这项研究是为了探索EGb761在实验诱导的溃疡中的治疗和预防益处。为了在小鼠中发展胃溃疡，口服给予吲哚美辛(40mg/kg)。在吲哚美辛给药之前（预防性）和之后（治疗性）通过管饲法给予EGb761（200mg/kg）7天。评估组织学改变和宏观粘膜病变。在胃组织匀浆中，丙二醛（MDA），还原型谷胱甘肽（GSH），一氧化氮（NO），和炎性细胞因子进行测量。环氧合酶-2(COX-2)的表达,细胞因子,还研究了胃粘膜中的增殖细胞核抗原（PCNA）。溃疡指数,组织学改变，胃氧化剂，消炎痛和炎症生物标志物均显著增加。在胃标本中，它增加了COX-2和PCNA的表达。EGB761治疗，预防和治疗，导致溃疡病变显著减少，亚硝基和氧化损伤，和炎症标志物，随着COX-2和PCNA表达的降低。此外，在对抗胃溃疡的斗争中，发现EGb761治疗比预防更有效。
