Abstract：
Ammonia (NH3) is a common air pollutant, which poses a serious threat to farm animals. L-selenomethionine is organic selenium (Se), which can inhibit intracellular ROS generation, block ROS-dependent autophagy, promote mitochondrial energy metabolism, and enhance the body\'s immunity. Lung, as an important organ of the respiratory system, is highly susceptible to the toxic effects of NH3. However, there were few studies on the mechanism of toxic effects of NH3 on lung tissues. The aim of this study was to investigate the effect of NH3 on the lungs in pigs and the alleviating effect of L-selenomethionine. Twenty-four Large White*Duroc*Min pigs were randomly assigned to 4 groups: control group, NH3 group, Se group, and NH3 +Se group. The results showed that exposure to NH3 caused damage and inflammation in lung tissues and significantly increased blood NH3 concentration. NH3 induced changes of oxidative stress indexes (GSH, GSH-Px, SOD, MDA, Keap1, Nrf2, and HO-1) and expressions of energy metabolism related genes (HK1, HK2, PFK, PK, LDHA, and HIF-1α). Ultrastructure showed that mitochondrial damage and autophagosome increased significantly, and the expression levels of autophagy related genes (Beclin1, ATG5, ATG7, ATG10, and p62) changed. However, the addition of L-selenomethionine alleviated the above changes, but there was still a significant difference compared with the control group (P < 0.05). This finding can provide a new evidence for mitigation of NH3 toxicity.
摘要：
氨（NH3）是一种常见的空气污染物，这对农场动物构成了严重威胁。L-硒代蛋氨酸是有机硒（Se），可以抑制细胞内ROS的产生，阻断ROS依赖性自噬，促进线粒体能量代谢，增强身体的免疫力。肺，作为呼吸系统的重要器官，极易受到NH3的毒性作用。然而,关于NH3对肺组织的毒性作用机制的研究很少。本研究的目的是研究NH3对猪肺的影响以及L-硒代蛋氨酸的缓解作用。将24头大白*Duroc*Min猪随机分为4组：对照组，NH3组,Se组,和NH3+Se基团。结果表明，暴露于NH3会引起肺组织的损伤和炎症，并显着增加血液中的NH3浓度。NH3诱导的氧化应激指标(GSH，GSH-Px,SOD,MDA,Keap1，Nrf2和HO-1）和能量代谢相关基因（HK1，HK2，PFK，PK,LDHA,和HIF-1α)。超微结构显示线粒体损伤和自噬体明显增加，自噬相关基因(Beclin1、ATG5、ATG7、ATG10和p62)的表达水平发生变化。然而,L-硒代蛋氨酸的加入缓解了上述变化，但与对照组相比仍有显著性差异(P<0.05)。这一发现可以为减轻NH3毒性提供新的证据。
