PDF(Pubmed)
Abstract：
Type 1 diabetes mellitus (T1DM) is a chronic disease represented by insulin-causing pancreatic β-cell disruption and hyperglycemia. Therefore, it is necessary to establish a variety of animal models of diabetes to study the pathogenesis and pathophysiology of it. However, there are few reports on the use of beagle dogs to establish an animal model of type 1 diabetes. This study aimed to explore a simple and feasible modeling method to establish a long-term and stable type 1 diabetes model in beagle dogs. Forty adult beagle dogs were randomly divided into control group and model group. After 24 h of fasting, streptozotocin (20 mg/kg) and alloxan (20 mg/kg) were injected through the cephalic vein. The second intravenous injection was given on the 4th day after the first injection. Insulin release testing was performed on the 7th day after the last intravenous injection. Fasting blood glucose and body weight were recorded monthly. Four months after the last injection, the serum fructosamine content and the ratio of glycated hemoglobin were detected. Then, the pancreatic tissue was harvested for histopathological examination. The results showed that the level of fasting blood glucose of the 16 dogs in the model group was consistently higher than 11.1 mmol/L for 4 consecutive months. Moreover, compared with the control group, the insulin release curve of the model group was flat with no increase. The body weight of the model group was significantly reduced, and the ratios of blood glucose, fructosamine, and glycosylated hemoglobin were significantly higher than those in the control group. Meanwhile, histopathological examination of the pancreas showed that the islet beta cells appeared to have vacuoles or even necrosis. In the model group, pancreatic β-cells were damaged and insulin release was reduced. These results suggest that the above modeling methods can induce long-term and stable type 1 diabetes models in beagle dogs.
摘要：
1型糖尿病（T1DM）是一种以胰岛素引起的胰腺β细胞破坏和高血糖为代表的慢性疾病。因此,为了研究糖尿病的发病机制和病理生理，有必要建立多种糖尿病动物模型。然而,关于使用比格犬建立1型糖尿病动物模型的报道很少。本研究旨在探索一种简单可行的建模方法,以建立比格犬1型糖尿病的长期稳定模型。将40只成年比格犬随机分为对照组和模型组。禁食24小时后，通过头静脉注射链脲佐菌素（20mg/kg）和四氧嘧啶（20mg/kg）。在第一次注射后第4天给予第二次静脉注射。在最后一次静脉注射后的第7天进行胰岛素释放测试。每月记录空腹血糖和体重。最后一次注射后四个月，检测血清果糖胺含量和糖化血红蛋白比值。然后，取胰腺组织进行组织病理学检查.结果显示,模型组16只犬的空腹血糖水平连续4个月持续高于11.1mmol/L。此外，与对照组相比，模型组胰岛素释放曲线平坦，无增加。模型组体重显著降低,和血糖的比率，果糖胺，糖化血红蛋白明显高于对照组。同时,胰腺组织病理学检查显示胰岛β细胞出现空泡甚至坏死。在模型组中，胰腺β细胞受损,胰岛素释放减少.这些结果表明，上述建模方法可以在比格犬中诱导长期稳定的1型糖尿病模型。
