Abstract：
The purpose of this study was to assess the accuracy, repeatability, and performance limits of in vivo Mirau ultrahigh axial resolution (UHR) line field spectral domain (LF-SD) optical coherence tomography (OCT) for the measurement of Bowman\'s and epithelial thickness, and to provide a reference range of these values for healthy corneas.
Volunteers with no history and evidence of corneal disease were included in this study. An in vivo graph search image segmentation of the central cornea was obtained at the normal interface vector orientation. The Mirau-UHR-LF-SD-OCT system used has an axial resolution down to 2.4 µm in air (1.7 µm in tissue), with an A-scan speed of 204.8 kHz and a signal to noise ratio (sensitivity) of 69 (83) dB.
Nine volunteers were included, one of whom wore contact lenses. The repeatability of mean Bowman\'s and epithelial thicknesses were 0.3 and 1.0 µm, respectively. The measured 95% population range for healthy in vivo thickness was 13.7 to 19.6 µm for the Bowman\'s layer, and 41.9 to 61.8 µm for the epithelial layer.
The measured thicknesses of Bowman\'s layer and the corneal epithelium using the Mirau-UHR-LF-SD-OCT were both accurate, with the range for healthy in vivo thicknesses matching prior confocal and OCT systems of varying axial resolutions, and repeatable, equaling the best value prior reported.
T1. Development of a commercially viable clinical UHR OCT technology, enabling accurate measurement and interpretation of Bowman\'s and epithelial layer thickness in clinical practice.
Volunteers with no history and evidence of corneal disease were included in this study. An in vivo graph search image segmentation of the central cornea was obtained at the normal interface vector orientation. The Mirau-UHR-LF-SD-OCT system used has an axial resolution down to 2.4 µm in air (1.7 µm in tissue), with an A-scan speed of 204.8 kHz and a signal to noise ratio (sensitivity) of 69 (83) dB.
Nine volunteers were included, one of whom wore contact lenses. The repeatability of mean Bowman\'s and epithelial thicknesses were 0.3 and 1.0 µm, respectively. The measured 95% population range for healthy in vivo thickness was 13.7 to 19.6 µm for the Bowman\'s layer, and 41.9 to 61.8 µm for the epithelial layer.
The measured thicknesses of Bowman\'s layer and the corneal epithelium using the Mirau-UHR-LF-SD-OCT were both accurate, with the range for healthy in vivo thicknesses matching prior confocal and OCT systems of varying axial resolutions, and repeatable, equaling the best value prior reported.
T1. Development of a commercially viable clinical UHR OCT technology, enabling accurate measurement and interpretation of Bowman\'s and epithelial layer thickness in clinical practice.
摘要：
这项研究的目的是评估准确性，重复性,和体内Mirau超高轴向分辨率（UHR）线场谱域（LF-SD）光学相干断层扫描（OCT）的性能极限，用于测量Bowman和上皮厚度，并为健康角膜提供这些值的参考范围。
本研究包括无角膜疾病病史和证据的志愿者。在正常界面矢量方向上获得了中央角膜的体内图搜索图像分割。使用的Mirau-UHR-LF-SD-OCT系统在空气中的轴向分辨率低至2.4µm（在组织中为1.7µm），A扫描速度为204.8kHz，信噪比（灵敏度）为69（83）dB。
包括9名志愿者，其中一人戴着隐形眼镜。平均Bowman's和上皮厚度的可重复性为0.3和1.0µm，分别。Bowman层的健康体内厚度测量的95％群体范围为13.7至19.6µm，上皮层为41.9至61.8µm。
使用Mirau-UHR-LF-SD-OCT测量的Bowman层和角膜上皮的厚度都是准确的，与健康的体内厚度范围相匹配的现有共焦和OCT系统的不同轴向分辨率，并且可重复，等于先前报告的最佳值。
T1。开发商业上可行的临床UHROCT技术，能够在临床实践中准确测量和解释鲍曼和上皮层厚度。
本研究包括无角膜疾病病史和证据的志愿者。在正常界面矢量方向上获得了中央角膜的体内图搜索图像分割。使用的Mirau-UHR-LF-SD-OCT系统在空气中的轴向分辨率低至2.4µm（在组织中为1.7µm），A扫描速度为204.8kHz，信噪比（灵敏度）为69（83）dB。
包括9名志愿者，其中一人戴着隐形眼镜。平均Bowman's和上皮厚度的可重复性为0.3和1.0µm，分别。Bowman层的健康体内厚度测量的95％群体范围为13.7至19.6µm，上皮层为41.9至61.8µm。
使用Mirau-UHR-LF-SD-OCT测量的Bowman层和角膜上皮的厚度都是准确的，与健康的体内厚度范围相匹配的现有共焦和OCT系统的不同轴向分辨率，并且可重复，等于先前报告的最佳值。
T1。开发商业上可行的临床UHROCT技术，能够在临床实践中准确测量和解释鲍曼和上皮层厚度。
