Abstract：
MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans.
摘要：
miRNA靶向治疗为精准癌症治疗带来了巨大的希望。研究miRNA表达变化对细胞凋亡的影响对评价miRNA靶向治疗和实现个体化治疗具有重要意义。在这项研究中,我们设计了一种双色荧光纳米传感器，该传感器由分子信标修饰的氧化石墨烯和肽组成。纳米传感器可以同时检测和成像活细胞中的miRNA-221和凋亡蛋白caspase-3。细胞内实验表明，纳米传感器可以成功地用于通过双色成像原位监测miRNA-221表达变化对细胞凋亡的影响。目前的策略可以为研究miRNA靶向治疗的可行性提供新的途径。筛选靶向miRNA的新抗癌药物并制定个性化治疗计划。
