%0 Journal Article
%T Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor.
%A Ren M
%A Chen Z
%A Ge C
%A Hu W
%A Wang N
%A Yang L
%A Luan M
%A Xu J
%A Ren M
%A Chen Z
%A Ge C
%A Hu W
%A Wang N
%A Yang L
%A Luan M
%A Xu J
%A Ren M
%A Chen Z
%A Ge C
%A Hu W
%A Wang N
%A Yang L
%A Luan M
%A Xu J
%J Biosensors (Basel)
%V 12
%N 7
%D Jun 2022 23
%M 35884247
%F 5.743
%R 10.3390/bios12070444
%X MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans.