{Reference Type}: Journal Article
{Title}: Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor.
{Author}: Ren M;Chen Z;Ge C;Hu W;Wang N;Yang L;Luan M;Xu J;Ren M;Chen Z;Ge C;Hu W;Wang N;Yang L;Luan M;Xu J;Ren M;Chen Z;Ge C;Hu W;Wang N;Yang L;Luan M;Xu J;
{Journal}: Biosensors (Basel)
{Volume}: 12
{Issue}: 7
{Year}: Jun 2022 23
{Factor}: 5.743
{DOI}: 10.3390/bios12070444
{Abstract}: MiRNA-targeted therapy holds great promise for precision cancer therapy. It is important to investigate the effect of changes in miRNA expression on apoptosis in order to evaluate miRNA-targeted therapy and achieve personalized therapy. In this study, we designed a dual-color fluorescent nanosensor consisting of grapheme oxide modified with a molecular beacon and peptide. The nanosensor can simultaneously detect and image miRNA-221 and apoptotic protein caspase-3 in living cells. Intracellular experiments showed that the nanosensor could be successfully applied for in situ monitoring of the effect of miRNA-221 expression changes on apoptosis by dual-color imaging. The current strategy could provide new avenues for investigating the feasibility of miRNA-targeted therapy, screening new anti-cancer drugs targeting miRNA and developing personalized treatment plans.