Abstract：
Aspidopterys obcordata vine is a Chinese Dai ethnic herb used to treat urolithiasis. However, the material basis and underlying mechanisms remain undefined. In this study, a 2.3 kD inulin-like A. obcordata fructan (AOFOS) was isolated by size exclusion column chromatography and characterized by ultrahigh-performance liquid chromatography-ion trap-time of flight mass spectrometry (UPLC-IT-TOF-MS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography mass spectrometry (GC-MS) and high-performance gel permeation chromatography (HGPC). In addition, AOFOS showed unique anti-urolithiasis activity in Drosophila kidney stone models. Mechanism study indicated that AOFOS reduced the size of calcium oxalate crystals by inhibiting the formation of large size crystals and the generation rate of calcium oxalate crystals as well as the crystal form conversion from calcium oxalate monohydrate (COM) to calcium oxalate dihydrate (COD).
摘要：
Aspidopterysobcordatavine是一种用于治疗尿石症的中国Dai族草药。然而,物质基础和潜在机制仍未定义。在这项研究中,通过尺寸排阻柱色谱分离出2.3kD菊粉样A.obcoradata果聚糖（AOFOS），并通过超高效液相色谱-离子阱-飞行时间质谱（UPLC-IT-TOF-MS）进行表征，核磁共振（NMR）光谱，气相色谱质谱(GC-MS)和高效凝胶渗透色谱(HGPC)。此外,AOFOS在果蝇肾结石模型中显示出独特的抗尿石症活性。机理研究表明，AOFOS通过抑制大尺寸晶体的形成和草酸钙晶体的生成速率以及从一水合草酸钙（COM）到二水合草酸钙（COD）的晶型转化来减小草酸钙晶体的尺寸。
