Abstract：
BACKGROUND: Acanthamoeba keratitis is a painful, sight-threatening infection. It is commonly associated with the use of contact lens. Several lines of evidence suggest inadequate contact lens solutions especially against the cyst forms of pathogenic Acanthamoeba, indicating the need to develop effective disinfectants.
OBJECTIVE: In this work, the application and assessment of montmorillonite clay (Mt-clay), cetylpyridinium chloride (CPC) and cetylpyridinium chloride-montmorillonite clay complex (CPC-Mt) against keratitis-causing A. castellanii belonging to the T4 genotype was studied.
METHODS: Adhesion to human cells and amoeba-mediated cytopathogenicity assays were conducted to determine the impact of Mt-clay, CPC and CPC-Mt complex on amoeba-mediated binding and host cell death. Furthermore, assays were also performed to determine inhibitory effects of Mt-clay, CPC and CPC-Mt complex on encystment and excystment. In addition, the cytotoxicity of Mt-clay, CPC and CPC-Mt complex against human cells was examined.
RESULTS: The results revealed that CPC and CPC-Mt complex presented significant antiamoebic effects against A. castellanii at microgram dose. Also, the CPC and CPC-Mt complex inhibited amoebae binding to host cells. Furthermore, CPC and CPC-Mt complex, were found to inhibit the encystment and excystment processes. Finally, CPC and CPC-Mt complex showed minimal host cell cytotoxicity. These results show that CPC and CPC-Mt complex exhibit potent anti-acanthamoebic properties.
CONCLUSIONS: Given the ease of usage, safety, cost-effectiveness and long-term stability, CPC and CPC-Mt complex can prove to be an excellent choice in the rational development of contact-lens disinfectants to eradicate pathogenic Acanthamoeba effectively.
OBJECTIVE: In this work, the application and assessment of montmorillonite clay (Mt-clay), cetylpyridinium chloride (CPC) and cetylpyridinium chloride-montmorillonite clay complex (CPC-Mt) against keratitis-causing A. castellanii belonging to the T4 genotype was studied.
METHODS: Adhesion to human cells and amoeba-mediated cytopathogenicity assays were conducted to determine the impact of Mt-clay, CPC and CPC-Mt complex on amoeba-mediated binding and host cell death. Furthermore, assays were also performed to determine inhibitory effects of Mt-clay, CPC and CPC-Mt complex on encystment and excystment. In addition, the cytotoxicity of Mt-clay, CPC and CPC-Mt complex against human cells was examined.
RESULTS: The results revealed that CPC and CPC-Mt complex presented significant antiamoebic effects against A. castellanii at microgram dose. Also, the CPC and CPC-Mt complex inhibited amoebae binding to host cells. Furthermore, CPC and CPC-Mt complex, were found to inhibit the encystment and excystment processes. Finally, CPC and CPC-Mt complex showed minimal host cell cytotoxicity. These results show that CPC and CPC-Mt complex exhibit potent anti-acanthamoebic properties.
CONCLUSIONS: Given the ease of usage, safety, cost-effectiveness and long-term stability, CPC and CPC-Mt complex can prove to be an excellent choice in the rational development of contact-lens disinfectants to eradicate pathogenic Acanthamoeba effectively.
摘要：
背景：棘阿米巴角膜炎是一种痛苦的，威胁视力的感染.它通常与隐形眼镜的使用有关。几条证据表明，隐形眼镜溶液不足，尤其是针对致病性棘阿米巴的囊肿形式，表明需要开发有效的消毒剂。
目的：在这项工作中，蒙脱石粘土(Mt-clay)的应用与评价，研究了氯化十六烷基吡啶（CPC）和氯化十六烷基吡啶-蒙脱石粘土复合物（CPC-Mt）对引起T4基因型角膜炎的A.castellanii。
方法：进行了对人类细胞的粘附和变形虫介导的细胞致病性测定，以确定Mt-clay的影响，CPC和CPC-Mt复合物对变形虫介导的结合和宿主细胞死亡的影响。此外，还进行了测定以确定Mt-clay的抑制作用，CPC和CPC-Mt复合体在封存和扩张上。此外,Mt-clay的细胞毒性，检查了针对人细胞的CPC和CPC-Mt复合物。
结果：结果显示，在微克剂量下，CPC和CPC-Mt复合物对A.castellanii具有显着的抗阿米巴作用。此外，CPC和CPC-Mt复合物抑制变形虫与宿主细胞的结合。此外，中国共产党和中国共产党山综合体，被发现抑制了包壳和包装过程。最后,CPC和CPC-Mt复合物显示最小的宿主细胞毒性。这些结果表明CPC和CPC-Mt复合物表现出有效的抗棘阿米巴特性。
结论：鉴于易于使用，安全,成本效益和长期稳定性，CPC和CPC-Mt复合物可以证明是合理开发隐形眼镜消毒剂以有效根除致病性棘阿米巴的绝佳选择。
目的：在这项工作中，蒙脱石粘土(Mt-clay)的应用与评价，研究了氯化十六烷基吡啶（CPC）和氯化十六烷基吡啶-蒙脱石粘土复合物（CPC-Mt）对引起T4基因型角膜炎的A.castellanii。
方法：进行了对人类细胞的粘附和变形虫介导的细胞致病性测定，以确定Mt-clay的影响，CPC和CPC-Mt复合物对变形虫介导的结合和宿主细胞死亡的影响。此外，还进行了测定以确定Mt-clay的抑制作用，CPC和CPC-Mt复合体在封存和扩张上。此外,Mt-clay的细胞毒性，检查了针对人细胞的CPC和CPC-Mt复合物。
结果：结果显示，在微克剂量下，CPC和CPC-Mt复合物对A.castellanii具有显着的抗阿米巴作用。此外，CPC和CPC-Mt复合物抑制变形虫与宿主细胞的结合。此外，中国共产党和中国共产党山综合体，被发现抑制了包壳和包装过程。最后,CPC和CPC-Mt复合物显示最小的宿主细胞毒性。这些结果表明CPC和CPC-Mt复合物表现出有效的抗棘阿米巴特性。
结论：鉴于易于使用，安全,成本效益和长期稳定性，CPC和CPC-Mt复合物可以证明是合理开发隐形眼镜消毒剂以有效根除致病性棘阿米巴的绝佳选择。
