Abstract：
Laboratory animal research involving mice, requires consideration of many factors to be controlled. Genetic quality is one factor that is often overlooked but is essential for the generation of reproducible experimental results. Whether experimental research involves inbred mice, spontaneous mutant, or genetically modified strains, exercising genetic quality through careful breeding, good recordkeeping, and prudent quality control steps such as validation of the presence of mutations and verification of the genetic background, will help ensure that experimental results are accurate and that reference controls are representative for the particular experiment. In this review paper, we will discuss various techniques used for the generation of genetically altered mice, and the different aspects to be considered regarding genetic quality, including inbred strains and substrains used, quality check controls during and after genetic manipulation and breeding. We also provide examples for when to use the different techniques and considerations on genetic quality checks. Further, we emphasize on the importance of establishing an in-house genetic quality program.
摘要：
涉及小鼠的实验动物研究，需要考虑许多因素来控制。遗传质量是一个经常被忽视的因素，但对于产生可重复的实验结果至关重要。实验研究是否涉及近交小鼠，自发突变,或转基因菌株，通过精心育种锻炼遗传质量，良好的记录保存，以及审慎的质量控制步骤，例如验证突变的存在和验证遗传背景，将有助于确保实验结果是准确的，并且参考对照对于特定实验具有代表性。在这篇综述论文中，我们将讨论用于产生转基因小鼠的各种技术，以及遗传质量需要考虑的不同方面，包括使用的近交系和子系，基因操作和育种期间和之后的质量检查控制。我们还提供了何时使用不同技术和遗传质量检查注意事项的示例。Further,我们强调建立内部遗传质量计划的重要性。
