背景:胃肠道运输(GIT)受饮食等因素的影响,药物,遗传学,和肠道微生物群,缓慢的GIT可能表明与疾病有关的功能障碍,如便秘。尽管GIT研究已经利用了各种动物模型,很少有效地模拟自发的缓慢GIT。
目的:我们旨在表征CFP/Yit(CFP)的GIT表型,具有建议的缓慢GIT的近交小鼠品系。
方法:将雌性和雄性CFP小鼠与GIT中的Crl:CD1(ICR)小鼠进行比较,并基于荧光标记的70kDa葡聚糖的口服管饲法进行评估,饲料摄入量,粪便量,和粪便含水量。进行结肠的组织病理学分析和肠道微生物群的分析。
结果:与ICR小鼠相比,CFP小鼠表现出较短的小肠和1.4倍的结肠。CFP小鼠的中位全GIT时间比ICR小鼠长6.0倍。CFP小鼠表现出比ICR小鼠更慢的胃和盲肠过境,结肠转运时间中位数为4.1小时(长2.9倍)。CFP小鼠表现出较低的每日饲料摄入量和粪便量。CFP小鼠的粪便含水量较低,显然归因于更长的结肠。组织病理学分析显示CFP小鼠无变化,包括肿瘤或炎症。此外,CFP小鼠的Firmicutes/拟杆菌比例较高,盲肠和粪便内容物中的丹毒菌科相对丰富。
结论:这项研究表明,CFP小鼠在胃中表现出缓慢的运输,盲肠,和结肠。作为一种新颖的小鼠模型,CFP小鼠可有助于胃肠生理和疾病的研究。
BACKGROUND: Gastrointestinal transit (GIT) is influenced by factors including diet, medications, genetics, and gut microbiota, with slow GIT potentially indicating a functional disorder linked to conditions, such as constipation. Although GIT studies have utilized various animal models, few effectively model spontaneous slow GIT.
OBJECTIVE: We aimed to characterize the GIT phenotype of CFP/Yit (CFP), an inbred mouse strain with suggested slow GIT.
METHODS: Female and male CFP mice were compared to Crl:CD1 (ICR) mice in GIT and assessed based on oral gavage of fluorescent-labeled 70-kDa dextran, feed intake, fecal amount, and fecal water content. Histopathological analysis of the colon and analysis of gut microbiota were conducted.
RESULTS: CFP mice exhibited a shorter small intestine and a 1.4-fold longer colon compared to ICR mice. The median whole-GIT time was 6.0-fold longer in CFP mice than in ICR mice. CFP mice demonstrated slower gastric and cecal transits than ICR mice, with a median colonic transit time of 4.1 h (2.9-fold longer). CFP mice exhibited lower daily feed intakes and fecal amounts. Fecal water content was lower in CFP mice, apparently attributed to the longer colon. Histopathological analysis showed no changes in CFP mice, including tumors or inflammation. Moreover, CFP mice had a higher Firmicutes/Bacteroidota ratio and a relative abundance of Erysipelotrichaceae in cecal and fecal contents.
CONCLUSIONS: This study indicates that CFP mice exhibit slow transit in the stomach, cecum, and colon. As a novel mouse model, CFP mice can contribute to the study of gastrointestinal physiology and disease.