Abstract：
Mineral lubricating oils are widely used in various industrial sectors for their applications in maintenance and functioning of machineries. However, indiscriminate dumping of these used oils have resulted in polluting the natural reservoirs which subsequently destroys ecological balance. Bacteria can emulsify or lower surface tension between phases of immiscible substrates and can acquire them as their carbon and energy sources. Such a phenomenon is mediated by production of extracellular polymers which can function as eminent surface active compounds based on their surfactant or emulsifying nature. The comparison between bacterial strains (Gram-positive Bacillus stratosphericus A15 and Gram-negative Ochrobactrum pseudintermedium C1) on utilization of pure straight chain hydrocarbons, waste mineral lubricating oils as sole carbon source and chemical characterization of the synthesized surface active compounds were studied. Characterization analysis by Ultraviolet Visible spectrophotometry, Fourier transform infrared spectroscopy, Nuclear Magnetic Resonance spectroscopy, Carbon-Hydrogen-Nitrogen analysis has given detailed structural elucidation of surface active compounds. The contrasting nature of bacterial strains in utilization of different hydrocarbons of waste mineral lubricating oils was observed in Gas Chromatography-Mass Spectroscopy analysis. The variation between both strains in utilization of hydrocarbons can be manifested in chemical structural differences and properties of the produced surface active compounds. Scanning Electron Microscopy has given detailed insight into the microstructural difference of the compounds. The utilization of lubricating oils can address waste disposal problem and offer an economical feasible approach for bacterial production of surface active compounds. Our results suggest that these surface active compounds can maneuver applications in environmental bioremediation and agriculture, pharmaceuticals and food as functional biomaterials.
摘要：
矿物润滑油广泛用于各种工业部门，因为它们在机器的维护和运行中的应用。然而,不分青红皂白地倾倒这些用过的油导致污染了天然水库，随后破坏了生态平衡。细菌可以乳化或降低不混溶底物的相之间的表面张力，并可以将其作为碳源和能源。这种现象是由细胞外聚合物的产生介导的,所述细胞外聚合物基于其表面活性剂或乳化性质可作为杰出的表面活性化合物起作用。细菌菌株（革兰氏阳性的平水芽孢杆菌A15和革兰氏阴性的假中间培养基C1）之间对纯直链烃利用的比较，研究了以废矿物润滑油为唯一碳源的合成表面活性化合物的化学表征。通过紫外可见分光光度法进行表征分析，傅里叶变换红外光谱,核磁共振波谱,碳-氢-氮分析给出了表面活性化合物的详细结构说明。在气相色谱-质谱分析中观察到细菌菌株在废矿物润滑油的不同烃利用中的对比性质。在烃的利用中,两种菌株之间的差异可以表现为所产生的表面活性化合物的化学结构差异和性质。扫描电子显微镜已详细了解了化合物的微观结构差异。润滑油的利用可以解决废物处理问题，并为细菌生产表面活性化合物提供经济可行的方法。我们的结果表明，这些表面活性化合物可以在环境生物修复和农业中进行应用。药物和食品作为功能性生物材料。
