Abstract：
To analyze the microbiological profile and in vitro antibiotic susceptibility patterns of bacterial isolates in canaliculitis, an infection of the lacrimal drainage system of the eye.
The laboratory records of patients presenting with canaliculitis from whom specimens were obtained for microbiological investigations at our tertiary eye care centre in South India from January 2010 to December 2020, were reviewed.
A total of 130 canalicular pus samples were collected from 112 patients and submitted for microbiological studies during the study period. A total of 183 micro-organisms were isolated from 115 culture-positive specimens. The micro-organisms isolated were predominantly aerobic Gram-positive bacteria (83.44%), with Coagulase-negative Staphylococci (CoNS/Other Staphylococcus spp) (31.69%), Corynebacterium spp (15.3%), Staphylococcus aureus (9.84%) and Viridans Streptococci (9.84%) accounting for a majority of the isolates. Actinomycesspp (6.56%) was the most common anaerobic bacterium isolated. Our study revealed several bacteria not previously associated with canaliculitis namely Ottowia spp, Elizabethkingiameningoseptica, Aeromonassalmonicida, Capnocytophagaochracea and Campylobacter gracilis. Polymicrobial aetiology was observed in 39.13% of culture-positive samples. Analysis of antibiotic susceptibility patterns of the isolates revealed a high proportion of Gram-positive bacteria susceptible to chloramphenicol (90.16%) compared to fluoroquinolones including ciprofloxacin (74.42%), norfloxacin (64.15%) and gatifloxacin (60.49%).
This study represents the largest series of canaliculitis reporting the microbiological profile and antibiotic susceptibilities of the isolated micro-organisms, till date. Gram-positive bacteria accounted for a majority of isolates, predominated by Staphylococcus spp. The increasing resistance of Gram-positive bacteria to fluoroquinolones warrants antibiotic treatment in canaliculitis is based on in vitro antimicrobial susceptibility patterns.
The laboratory records of patients presenting with canaliculitis from whom specimens were obtained for microbiological investigations at our tertiary eye care centre in South India from January 2010 to December 2020, were reviewed.
A total of 130 canalicular pus samples were collected from 112 patients and submitted for microbiological studies during the study period. A total of 183 micro-organisms were isolated from 115 culture-positive specimens. The micro-organisms isolated were predominantly aerobic Gram-positive bacteria (83.44%), with Coagulase-negative Staphylococci (CoNS/Other Staphylococcus spp) (31.69%), Corynebacterium spp (15.3%), Staphylococcus aureus (9.84%) and Viridans Streptococci (9.84%) accounting for a majority of the isolates. Actinomycesspp (6.56%) was the most common anaerobic bacterium isolated. Our study revealed several bacteria not previously associated with canaliculitis namely Ottowia spp, Elizabethkingiameningoseptica, Aeromonassalmonicida, Capnocytophagaochracea and Campylobacter gracilis. Polymicrobial aetiology was observed in 39.13% of culture-positive samples. Analysis of antibiotic susceptibility patterns of the isolates revealed a high proportion of Gram-positive bacteria susceptible to chloramphenicol (90.16%) compared to fluoroquinolones including ciprofloxacin (74.42%), norfloxacin (64.15%) and gatifloxacin (60.49%).
This study represents the largest series of canaliculitis reporting the microbiological profile and antibiotic susceptibilities of the isolated micro-organisms, till date. Gram-positive bacteria accounted for a majority of isolates, predominated by Staphylococcus spp. The increasing resistance of Gram-positive bacteria to fluoroquinolones warrants antibiotic treatment in canaliculitis is based on in vitro antimicrobial susceptibility patterns.
摘要：
分析小管炎中细菌分离株的微生物学特征和体外抗生素敏感性模式。眼睛泪道引流系统的感染。
回顾了2010年1月至2020年12月在印度南部三级眼科护理中心获得标本进行微生物学研究的泪小管炎患者的实验室记录。
在研究期间，从112名患者中收集了总共130个泪管脓液样本，并提交了微生物学研究。从115个培养阳性标本中分离出183个微生物。分离的微生物主要为需氧菌革兰氏阳性菌(83.44%),凝固酶阴性葡萄球菌（CoNS/其他葡萄球菌）（31.69％），棒状杆菌属（15.3%），金黄色葡萄球菌（9.84％）和Viridans链球菌（9.84％）占大多数。放线菌(6.56%)是最常见的厌氧菌。我们的研究揭示了几种以前与小管炎无关的细菌，即Ottowiaspp，Elizabethkingameningospica,Aeromonassalmonicida,Capnocytophagaochracea和gracilis弯曲杆菌。在39.13％的培养阳性样品中观察到多微生物病因。对分离株的抗生素敏感性模式的分析表明，与包括环丙沙星在内的氟喹诺酮类药物（74.42％）相比，对氯霉素敏感的革兰氏阳性菌比例较高（90.16％），诺氟沙星(64.15%)和加替沙星(60.49%)。
这项研究代表了最大的泪小管炎系列，报告了分离的微生物的微生物学特征和抗生素敏感性，直到日期。革兰氏阳性菌占分离株的大部分，以葡萄球菌属为主。革兰氏阳性细菌对氟喹诺酮类药物的耐药性不断增加，这保证了在泪小管炎中的抗生素治疗是基于体外抗菌药物敏感性模式。
回顾了2010年1月至2020年12月在印度南部三级眼科护理中心获得标本进行微生物学研究的泪小管炎患者的实验室记录。
在研究期间，从112名患者中收集了总共130个泪管脓液样本，并提交了微生物学研究。从115个培养阳性标本中分离出183个微生物。分离的微生物主要为需氧菌革兰氏阳性菌(83.44%),凝固酶阴性葡萄球菌（CoNS/其他葡萄球菌）（31.69％），棒状杆菌属（15.3%），金黄色葡萄球菌（9.84％）和Viridans链球菌（9.84％）占大多数。放线菌(6.56%)是最常见的厌氧菌。我们的研究揭示了几种以前与小管炎无关的细菌，即Ottowiaspp，Elizabethkingameningospica,Aeromonassalmonicida,Capnocytophagaochracea和gracilis弯曲杆菌。在39.13％的培养阳性样品中观察到多微生物病因。对分离株的抗生素敏感性模式的分析表明，与包括环丙沙星在内的氟喹诺酮类药物（74.42％）相比，对氯霉素敏感的革兰氏阳性菌比例较高（90.16％），诺氟沙星(64.15%)和加替沙星(60.49%)。
这项研究代表了最大的泪小管炎系列，报告了分离的微生物的微生物学特征和抗生素敏感性，直到日期。革兰氏阳性菌占分离株的大部分，以葡萄球菌属为主。革兰氏阳性细菌对氟喹诺酮类药物的耐药性不断增加，这保证了在泪小管炎中的抗生素治疗是基于体外抗菌药物敏感性模式。
