Abstract：
Hydrogels have been recently proposed as suitable materials to generate reactive oxygen and nitrogen species (RONS) upon gas-plasma treatment, and postulated as promising alternatives to conventional cancer therapies. Acting as delivery vehicles that allow a controlled release of RONS to the diseased site, plasma-treated hydrogels can overcome some of the limitations presented by plasma-treated liquids in in vivo therapies. In this work, we optimized the composition of a methylcellulose (MC) hydrogel to confer it with the ability to form a gel at physiological temperatures while remaining in the liquid phase at room temperature to allow gas-plasma treatment with suitable formation of plasma-generated RONS. MC hydrogels demonstrated the capacity for generation, prolonged storage and release of RONS. This release induced cytotoxic effects on the osteosarcoma cancer cell line MG-63, reducing its cell viability in a dose-response manner. These promising results postulate plasma-treated thermosensitive hydrogels as good candidates to provide local anticancer therapies.
摘要：
最近已经提出水凝胶作为在气体等离子体处理时产生活性氧和氮物质(RONS)的合适材料。并被认为是传统癌症疗法的有希望的替代品。作为运载工具，允许RONS受控释放到患病部位，等离子体处理的水凝胶可以克服由等离子体处理的液体在体内治疗中呈现的一些限制。在这项工作中,我们优化了甲基纤维素（MC）水凝胶的组成，使其具有在生理温度下形成凝胶的能力，同时在室温下保持在液相中，以允许在适当形成等离子体产生的RONS的情况下进行气体等离子体处理。MC水凝胶展示了产生的能力，延长RONS的储存和释放。这种释放对骨肉瘤癌细胞系MG-63诱导了细胞毒性作用，以剂量反应的方式降低了其细胞活力。这些有希望的结果假定等离子体处理的热敏水凝胶是提供局部抗癌疗法的良好候选物。
