Abstract：
BACKGROUND: The purpose of this pilot study was to provide information about the washout-dependent depletion of important skin components in the horny layer of the scalp. They were taken as markers for scalp drying effects of cosmetic cleansing products and were measured directly in vivo.
METHODS: In vivo confocal Raman spectroscopy was used to measure the depletion of the total natural moisturizing factor (total NMF) and some of its components (urea and lactic acid) as well as a fraction of stratum corneum lipids, after repeated washing with a standard shampoo on the human scalp.
RESULTS: The measurements showed a reduction in the amount of NMF and lipids of the stratum corneum caused by repeated shampooing.
CONCLUSIONS: Confocal Raman spectroscopy is an innovative technology that can be used successfully in vivo on the hairy scalp. The loss of the most important skin components caused by hair washing can be quantified directly with this technology. The method is valuable to support the development cosmetic cleansing products, as it is suitable to directly compare the effects of different product candidates on the human scalp in a most realistic way.
METHODS: In vivo confocal Raman spectroscopy was used to measure the depletion of the total natural moisturizing factor (total NMF) and some of its components (urea and lactic acid) as well as a fraction of stratum corneum lipids, after repeated washing with a standard shampoo on the human scalp.
RESULTS: The measurements showed a reduction in the amount of NMF and lipids of the stratum corneum caused by repeated shampooing.
CONCLUSIONS: Confocal Raman spectroscopy is an innovative technology that can be used successfully in vivo on the hairy scalp. The loss of the most important skin components caused by hair washing can be quantified directly with this technology. The method is valuable to support the development cosmetic cleansing products, as it is suitable to directly compare the effects of different product candidates on the human scalp in a most realistic way.
摘要：
背景：这项初步研究的目的是提供有关头皮角质层中重要皮肤成分的冲洗依赖性消耗的信息。它们被用作化妆品清洁产品的头皮干燥效果的标记，并在体内直接测量。
方法：体内共聚焦拉曼光谱用于测量总天然保湿因子（总NMF）及其某些成分（尿素和乳酸）以及一部分角质层脂质的消耗，用标准洗发水反复洗涤后，在人的头皮上。
结果:测量显示由反复洗发引起的角质层的NMF和脂质的量减少。
结论：共聚焦拉曼光谱是一项创新技术，可在毛茸茸的头皮上成功用于体内。由洗发引起的最重要的皮肤成分的损失可以通过该技术直接量化。该方法对支持化妆品清洁产品的开发很有价值，因为它适合以最现实的方式直接比较不同候选产品对人类头皮的影响。
方法：体内共聚焦拉曼光谱用于测量总天然保湿因子（总NMF）及其某些成分（尿素和乳酸）以及一部分角质层脂质的消耗，用标准洗发水反复洗涤后，在人的头皮上。
结果:测量显示由反复洗发引起的角质层的NMF和脂质的量减少。
结论：共聚焦拉曼光谱是一项创新技术，可在毛茸茸的头皮上成功用于体内。由洗发引起的最重要的皮肤成分的损失可以通过该技术直接量化。该方法对支持化妆品清洁产品的开发很有价值，因为它适合以最现实的方式直接比较不同候选产品对人类头皮的影响。
