尽管对二苯甲酮-3(BP3)的大量实验表明它可以渗透到皮肤中,体内渗透情况和尿代谢趋势尚未研究。在这项研究中,选择含有5.72%BP3的防晒霜用于人体皮肤暴露。共聚焦拉曼被成功地用于研究BP3在防晒剂中的体内皮肤渗透。在皮肤暴露2小时内,使用防晒霜15、30、60和120分钟后,半定量平均值分别为5.50、13.48、15.79和15.00μg/cm2,分别,表明BP3在60-120分钟内穿透角质层。在人类四肢暴露一次后,BP3迅速代谢并通过尿液排出,并在第6小时达到峰值浓度,而其代谢物2,4-二羟基二苯甲酮(BP1)在第9小时达到峰值浓度。同时,6%BP3和1%BP1在48h内通过尿液排泄,但2,2'-二羟基-4-甲氧基二苯甲酮(BP8)的浓度较低,尽管它在暴露后48小时内变化很大。在连续曝光期间,发现BP3浓度与暴露时间之间存在显着相关性(p<0.05),表明BP3浓度在更长的暴露时间增加。因此,拉曼光谱与人体样品分析相结合,为评估个人护理添加剂在人体内的吸收和代谢提供了新的途径。
Although numerous experiments on benzophenone-3 (BP3) have shown that it can permeate into the skin, the in vivo penetration situation and urinary metabolic trend have not yet been investigated. In this study, sunscreen containing 5.72% BP3 was selected for human-skin exposure. Confocal Raman was successfully used to investigate in vivo skin penetration of BP3 in sunscreen. During 2 h of skin exposure, the semi-quantitative mean values were 5.50, 13.48, 15.79, and 15.00 μg/cm2 after application of sunscreen for 15, 30, 60, and 120 min, respectively, indicating that BP3 penetrated the stratum corneum during 60-120 min. After a single exposure of human limbs, BP3 was quickly metabolized and excreted through urine and reached its peak concentration in the 6th hour, whereas its metabolite 2,4-dihydroxybenzophenone (BP1) reached its peak concentration in the 9th hour. Meanwhile, 6% BP3 and 1% BP1 were excreted through the urine within 48 h, but the concentration of 2,2\'-dihydroxy-4-methoxybenzophenone (BP8) was low, although it varied greatly within 48 h after exposure. During consecutive exposures, a significant correlation (p < 0.05) between BP3 concentration and exposure time was found, indicating that BP3 concentration increased at longer exposure times. Therefore, combining Raman spectroscopy and human sample analysis provided a new way to assess absorption and metabolism of personal care additives in the human body.