We collected 36,927 samples (ages ranged from 1 day to 101 years old) from cases with acute respiratory tract infections (ARTI) using the Respiratory Pathogens Surveillance System (RPSS) in Beijing, 2015-2019. G gene sequencing and phylogenetic analysis were performed to identify RSV genotypes, clusters, and amino acid (aa) changes.
In total, 764 (2.1%, 764/36927) cases were RSV positive, 52.1% cases were children under 5 years old, and 25.8% were elderly ≥ 60 years old. We obtained 369 sequences of the G gene. ON1 and BA9 were the dominant genotypes in Beijing. Sub-lineage 4 of ON1, which contains four aa substitutions (T113I, N178G, H258Q, and H266L), emerged in 2017 and became the predominant variant in 2018-2019. Sub-lineage 4 of BA9, which contains two aa changes (A131T, T137I), emerged in 2017 and became the predominant variant in 2019. We also observed 10 rarely reported nucleotide deletions in the 3\' end of the G gene from five sequences of the ON1 genotype.
With the exception of children < 5 years old, RSV infection mainly occurred in the elderly ≥ 60 years old. Newly emerged sub-lineages have replaced existing sub-lineage over time and become predominant in Beijing. Continued surveillance of the genetic diversity of RSV is necessary.
我们在北京使用呼吸病原体监测系统(RPSS)从急性呼吸道感染(ARTI)病例中收集了36,927个样本(年龄从1天到101岁不等),2015-2019年。进行G基因测序和系统发育分析以鉴定RSV基因型,集群,氨基酸(aa)的变化。
总共,764(2.1%,764/36927)例RSV阳性,52.1%为5岁以下儿童,≥60岁的老年人占25.8%。我们获得了G基因的369个序列。ON1和BA9是北京的优势基因型。ON1的子谱系4,包含四个aa取代(T113I,N178G,H258Q,和H266L),出现于2017年,并在2018-2019年成为主要变体。BA9的子谱系4,其中包含两个aa变化(A131T,T137I),出现于2017年,并在2019年成为主要变体。我们还观察到来自ON1基因型的五个序列的G基因3'末端的10个很少报道的核苷酸缺失。
除5岁以下儿童外,RSV感染主要发生在≥60岁的老年人。随着时间的推移,新出现的亚谱系已经取代了现有的亚谱系,并在北京占据主导地位。持续监测RSV的遗传多样性是必要的。