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Abstract：
The NLRP3 gene is reportedly associated with several autoimmune diseases. However, in the Chinese Han population, whether NLRP3 polymorphisms are associated with type 1 diabetes (T1D) is unclear. Therefore, this study examined the associations of rs3806265 and rs4612666 of the NLRP3 gene with T1D susceptibility and the clinical characteristics of Chinese Han T1D patients.
In total, 510 classic T1D patients and 531 healthy controls from the Chinese Han population were recruited for a case-control study. rs3806265 and rs4612666 of the NLRP3 gene were genotyped by MassARRAY. Logistic regression analysis and the chi-square test were used to compare the distributions of the alleles and genotypes of rs3806265 and rs4612666. The relationships between rs3806265 and rs4612666 and the clinical characteristics of T1D patients were analyzed by Kruskal-Wallis one-way ANOVA. Student\'s t test was used to analyze normally distributed data. Bonferroni correction was used for multiple comparisons.
1) rs3806265 was associated with glutamic acid decarboxylase antibody (GADA) titers (P = 0.02), and patients with the CC genotype had higher GADA titers than patients with the TT genotype. 2) rs4612666 was also associated with GADA titers (P=0.041). Compared with patients with the CC genotype, patients with the TT genotype had higher GADA titers. 3) rs3806265 and rs4612666 of the NLRP3 gene were not significantly associated with T1D susceptibility under different genetic models.
rs3806265 and rs4612666 of the NLRP3 gene were significantly associated with GADA titers in Chinese Han T1D patients.
In total, 510 classic T1D patients and 531 healthy controls from the Chinese Han population were recruited for a case-control study. rs3806265 and rs4612666 of the NLRP3 gene were genotyped by MassARRAY. Logistic regression analysis and the chi-square test were used to compare the distributions of the alleles and genotypes of rs3806265 and rs4612666. The relationships between rs3806265 and rs4612666 and the clinical characteristics of T1D patients were analyzed by Kruskal-Wallis one-way ANOVA. Student\'s t test was used to analyze normally distributed data. Bonferroni correction was used for multiple comparisons.
1) rs3806265 was associated with glutamic acid decarboxylase antibody (GADA) titers (P = 0.02), and patients with the CC genotype had higher GADA titers than patients with the TT genotype. 2) rs4612666 was also associated with GADA titers (P=0.041). Compared with patients with the CC genotype, patients with the TT genotype had higher GADA titers. 3) rs3806265 and rs4612666 of the NLRP3 gene were not significantly associated with T1D susceptibility under different genetic models.
rs3806265 and rs4612666 of the NLRP3 gene were significantly associated with GADA titers in Chinese Han T1D patients.
摘要：
据报道，NLRP3基因与几种自身免疫性疾病相关。然而,在中国汉族人口中，NLRP3多态性是否与1型糖尿病(T1D)相关尚不清楚.因此,本研究探讨了NLRP3基因rs3806265和rs4612666与中国汉族T1D患者的T1D易感性和临床特征的关系。
总共，从中国汉族人群中招募510名经典T1D患者和531名健康对照进行病例对照研究。通过MassARRAY对NLRP3基因的rs3806265和rs4612666进行基因分型。采用Logistic回归分析和卡方检验比较rs3806265和rs4612666的等位基因和基因型分布。采用Kruskal-Wallis单因素方差分析分析rs3806265和rs4612666与T1D患者临床特征的关系。采用t检验对正态分布数据进行分析。Bonferroni校正用于多重比较。
1)rs3806265与谷氨酸脱羧酶抗体(GADA)滴度相关(P=0.02),CC基因型患者的GADA滴度高于TT基因型患者。2)rs4612666也与GADA滴度相关(P=0.041)。与CC基因型患者相比，TT基因型患者的GADA滴度较高.3)NLRP3基因rs3806265和rs4612666在分歧遗传模子下与T1D易感性无显著相干性。
在中国汉族T1D患者中，NLRP3基因的rs3806265和rs4612666与GADA滴度显著相关。
总共，从中国汉族人群中招募510名经典T1D患者和531名健康对照进行病例对照研究。通过MassARRAY对NLRP3基因的rs3806265和rs4612666进行基因分型。采用Logistic回归分析和卡方检验比较rs3806265和rs4612666的等位基因和基因型分布。采用Kruskal-Wallis单因素方差分析分析rs3806265和rs4612666与T1D患者临床特征的关系。采用t检验对正态分布数据进行分析。Bonferroni校正用于多重比较。
1)rs3806265与谷氨酸脱羧酶抗体(GADA)滴度相关(P=0.02),CC基因型患者的GADA滴度高于TT基因型患者。2)rs4612666也与GADA滴度相关(P=0.041)。与CC基因型患者相比，TT基因型患者的GADA滴度较高.3)NLRP3基因rs3806265和rs4612666在分歧遗传模子下与T1D易感性无显著相干性。
在中国汉族T1D患者中，NLRP3基因的rs3806265和rs4612666与GADA滴度显著相关。
