METHODS: Allicin-coated silicone tracheal tube (t-tube) was prepared by the polydopamine-mediated coating method. Tracheal mucosa was injured, and an allicin-coated t-tube was placed into the trachea to evaluate mucosal changes until designated time point. Anti-inflammatory, anti-bacterial and cytotoxic effects of allicin were also investigated in in vitro.
RESULTS: Allicin- coated silicone was not cytotoxic, and it showed anti-inflammatory and anti-bacterial effects in in vitro analysis. The use of allicin-coated t-tube in a rabbit model showed favorable mucosal healing with significant decrease of proinflammatory cytokines compared to the non-coated tube group. The allicin-coated tube showed obvious decreased number of cocci-shaped bacterial attached to the tube surface. From the histological point of view, the allicin- coated tube showed faster regeneration of the normal respiratory epithelial structure compared to the non-coated group.
CONCLUSIONS: Allicin-coated t-tube showed anti-inflammatory and anti-bacterial effects on injured tracheal mucosa. We suggest that allicin-coated t-tube can be used for promoting physiological wound healing to prevent laryngotracheal stenosis.
方法:通过聚多巴胺介导的涂层方法制备了大蒜素涂层的有机硅气管导管(t-tube)。气管粘膜受伤,并将涂有大蒜素的t管置于气管中以评估粘膜变化直至指定时间点。抗炎,大蒜素的抗菌和细胞毒性作用也在体外进行了研究。
结果:Allicin-coatedsiliconewasnotcellular,在体外分析中显示了抗炎和抗菌作用。与未涂覆的管组相比,在兔模型中使用大蒜素涂覆的t管显示出良好的粘膜愈合,促炎细胞因子显着降低。大蒜素涂层的管显示附着在管表面的球菌状细菌数量明显减少。从组织学的角度来看,与未涂覆组相比,大蒜素涂覆的管显示正常呼吸上皮结构的更快再生。
结论:大蒜素涂层的t管对损伤的气管粘膜具有抗炎和抗菌作用。我们建议大蒜素涂层的t管可用于促进生理性伤口愈合,以防止喉气管狭窄。