Abstract：
OBJECTIVE: Allicin has been known to improve wound healing via antimicrobial and anti-inflammatory properties. The aim of this study was to evaluate whether an allicin-coated tracheal tube can prevent tracheal stenosis through improving wound healing after tracheal injury.
METHODS: Allicin-coated silicone tracheal tube (t-tube) was prepared by the polydopamine-mediated coating method. Tracheal mucosa was injured, and an allicin-coated t-tube was placed into the trachea to evaluate mucosal changes until designated time point. Anti-inflammatory, anti-bacterial and cytotoxic effects of allicin were also investigated in in vitro.
RESULTS: Allicin- coated silicone was not cytotoxic, and it showed anti-inflammatory and anti-bacterial effects in in vitro analysis. The use of allicin-coated t-tube in a rabbit model showed favorable mucosal healing with significant decrease of proinflammatory cytokines compared to the non-coated tube group. The allicin-coated tube showed obvious decreased number of cocci-shaped bacterial attached to the tube surface. From the histological point of view, the allicin- coated tube showed faster regeneration of the normal respiratory epithelial structure compared to the non-coated group.
CONCLUSIONS: Allicin-coated t-tube showed anti-inflammatory and anti-bacterial effects on injured tracheal mucosa. We suggest that allicin-coated t-tube can be used for promoting physiological wound healing to prevent laryngotracheal stenosis.
METHODS: Allicin-coated silicone tracheal tube (t-tube) was prepared by the polydopamine-mediated coating method. Tracheal mucosa was injured, and an allicin-coated t-tube was placed into the trachea to evaluate mucosal changes until designated time point. Anti-inflammatory, anti-bacterial and cytotoxic effects of allicin were also investigated in in vitro.
RESULTS: Allicin- coated silicone was not cytotoxic, and it showed anti-inflammatory and anti-bacterial effects in in vitro analysis. The use of allicin-coated t-tube in a rabbit model showed favorable mucosal healing with significant decrease of proinflammatory cytokines compared to the non-coated tube group. The allicin-coated tube showed obvious decreased number of cocci-shaped bacterial attached to the tube surface. From the histological point of view, the allicin- coated tube showed faster regeneration of the normal respiratory epithelial structure compared to the non-coated group.
CONCLUSIONS: Allicin-coated t-tube showed anti-inflammatory and anti-bacterial effects on injured tracheal mucosa. We suggest that allicin-coated t-tube can be used for promoting physiological wound healing to prevent laryngotracheal stenosis.
摘要：
目的：已知大蒜素通过抗菌和抗炎特性改善伤口愈合。这项研究的目的是评估大蒜素涂层的气管导管是否可以通过改善气管损伤后的伤口愈合来预防气管狭窄。
方法：通过聚多巴胺介导的涂层方法制备了大蒜素涂层的有机硅气管导管（t-tube）。气管粘膜受伤,并将涂有大蒜素的t管置于气管中以评估粘膜变化直至指定时间点。抗炎,大蒜素的抗菌和细胞毒性作用也在体外进行了研究。
结果：Allicin-coatedsiliconewasnotcellular,在体外分析中显示了抗炎和抗菌作用。与未涂覆的管组相比，在兔模型中使用大蒜素涂覆的t管显示出良好的粘膜愈合，促炎细胞因子显着降低。大蒜素涂层的管显示附着在管表面的球菌状细菌数量明显减少。从组织学的角度来看,与未涂覆组相比,大蒜素涂覆的管显示正常呼吸上皮结构的更快再生。
结论：大蒜素涂层的t管对损伤的气管粘膜具有抗炎和抗菌作用。我们建议大蒜素涂层的t管可用于促进生理性伤口愈合，以防止喉气管狭窄。
方法：通过聚多巴胺介导的涂层方法制备了大蒜素涂层的有机硅气管导管（t-tube）。气管粘膜受伤,并将涂有大蒜素的t管置于气管中以评估粘膜变化直至指定时间点。抗炎,大蒜素的抗菌和细胞毒性作用也在体外进行了研究。
结果：Allicin-coatedsiliconewasnotcellular,在体外分析中显示了抗炎和抗菌作用。与未涂覆的管组相比，在兔模型中使用大蒜素涂覆的t管显示出良好的粘膜愈合，促炎细胞因子显着降低。大蒜素涂层的管显示附着在管表面的球菌状细菌数量明显减少。从组织学的角度来看,与未涂覆组相比,大蒜素涂覆的管显示正常呼吸上皮结构的更快再生。
结论：大蒜素涂层的t管对损伤的气管粘膜具有抗炎和抗菌作用。我们建议大蒜素涂层的t管可用于促进生理性伤口愈合，以防止喉气管狭窄。
