Abstract：
Human pegivirus-1 (HPgV-1) is a lymphotropic human virus, typically considered nonpathogenic, but its infection can sometimes cause persistent viremia both in immunocompetent and immunosuppressed individuals. In a viral discovery research program in hematopoietic stem cell transplant (HSCT) pediatric patients, HPgV-1 was detected in 3 out of 14 patients (21.4%) using a target enrichment next-generation sequencing method, and the presence of the viruses was confirmed by agent-specific qRT-PCR assays. For the first time in this patient cohort, complete genomes of HPgV-1 were acquired and characterized. Phylogenetic analyses indicated that two patients had HPgV-1 genotype 2 and one had HPgV-1 genotype 3. Intra-host genomic variations were described and discussed. Our results highlight the necessity to screen HSCT patients and blood and stem cell donors to reduce the potential risk of HPgV-1 transmission.
摘要：
人类pegivirus-1（HPgV-1）是一种嗜淋巴细胞的人类病毒，通常被认为是非致病性的，但它的感染有时会导致免疫活性和免疫抑制个体的持续性病毒血症。在一项针对造血干细胞移植（HSCT）儿科患者的病毒发现研究计划中，使用靶标富集下一代测序方法在14例患者中的3例（21.4％）中检测到HPgV-1，病毒的存在通过试剂特异性qRT-PCR测定来确认。在这个患者队列中，第一次，获得并表征了HPgV-1的完整基因组。系统发育分析表明，两名患者的HPgV-1基因型为2，一名患者的HPgV-1基因型为3。描述并讨论了宿主内基因组变异。我们的结果强调了筛查HSCT患者以及血液和干细胞供体以降低HPgV-1传播的潜在风险的必要性。
