{Reference Type}: Journal Article
{Title}: Target Enrichment Metagenomics Reveals Human Pegivirus-1 in Pediatric Hematopoietic Stem Cell Transplantation Recipients.
{Author}: Ludowyke N;Phumiphanjarphak W;Apiwattanakul N;Manopwisedjaroen S;Pakakasama S;Sensorn I;Pasomsub E;Chantratita W;Hongeng S;Aiewsakun P;Thitithanyanont A;
{Journal}: Viruses
{Volume}: 14
{Issue}: 4
{Year}: 04 2022 12
{Factor}: 5.818
{DOI}: 10.3390/v14040796
{Abstract}: Human pegivirus-1 (HPgV-1) is a lymphotropic human virus, typically considered nonpathogenic, but its infection can sometimes cause persistent viremia both in immunocompetent and immunosuppressed individuals. In a viral discovery research program in hematopoietic stem cell transplant (HSCT) pediatric patients, HPgV-1 was detected in 3 out of 14 patients (21.4%) using a target enrichment next-generation sequencing method, and the presence of the viruses was confirmed by agent-specific qRT-PCR assays. For the first time in this patient cohort, complete genomes of HPgV-1 were acquired and characterized. Phylogenetic analyses indicated that two patients had HPgV-1 genotype 2 and one had HPgV-1 genotype 3. Intra-host genomic variations were described and discussed. Our results highlight the necessity to screen HSCT patients and blood and stem cell donors to reduce the potential risk of HPgV-1 transmission.