%0 Journal Article
%T Target Enrichment Metagenomics Reveals Human Pegivirus-1 in Pediatric Hematopoietic Stem Cell Transplantation Recipients.
%A Ludowyke N
%A Phumiphanjarphak W
%A Apiwattanakul N
%A Manopwisedjaroen S
%A Pakakasama S
%A Sensorn I
%A Pasomsub E
%A Chantratita W
%A Hongeng S
%A Aiewsakun P
%A Thitithanyanont A
%J Viruses
%V 14
%N 4
%D 04 2022 12
%M 35458526
%F 5.818
%R 10.3390/v14040796
%X Human pegivirus-1 (HPgV-1) is a lymphotropic human virus, typically considered nonpathogenic, but its infection can sometimes cause persistent viremia both in immunocompetent and immunosuppressed individuals. In a viral discovery research program in hematopoietic stem cell transplant (HSCT) pediatric patients, HPgV-1 was detected in 3 out of 14 patients (21.4%) using a target enrichment next-generation sequencing method, and the presence of the viruses was confirmed by agent-specific qRT-PCR assays. For the first time in this patient cohort, complete genomes of HPgV-1 were acquired and characterized. Phylogenetic analyses indicated that two patients had HPgV-1 genotype 2 and one had HPgV-1 genotype 3. Intra-host genomic variations were described and discussed. Our results highlight the necessity to screen HSCT patients and blood and stem cell donors to reduce the potential risk of HPgV-1 transmission.