Abstract:
The review discusses the role that proteins interacting with the translation termination factors eRF1 and eRF3 play in the control of protein synthesis and prionization. These proteins interact not only with each other, but also with many other proteins involved in controlling the efficiency of translation termination, and associate translation termination with other cell processes. The termination of translation is directly related not only to translation re-initiation and ribosome recycling, but also to mRNA stability and protein quality control. This connection is ensured by the interaction of eRF1 and eRF3 with proteins participating in various cell metabolic processes, such as mRNA transport from the nucleus into the cytoplasm (Dbp5/DDX19 and Gle1), ribosome recycling (Rli1/ABCE1), mRNA degradation (Upf proteins), and translation initiation (Pab1/PABP). In addition to genetic control, there is epigenetic control of translation termination. This mechanism is associated with prion polymerization of the Sup35 protein to form the [PSI^(+)] prion. The maintenance of the [PSI^(+)] prion, like other yeast prions, requires the operation of a system of molecular chaperones and protein sorting factors. The review considers in detail the interaction of the translation termination factors with proteins involved in various cellular processes.
摘要:
本文讨论了与翻译终止因子eRF1和eRF3相互作用的蛋白质在控制蛋白质合成和转录中的作用。这些蛋白质不仅相互作用,而且还有许多其他蛋白质参与控制翻译终止的效率,并将翻译终止与其他单元格进程相关联。翻译的终止不仅直接关系到翻译的重新启动和核糖体的循环,而且对mRNA的稳定性和蛋白质的质量控制。eRF1和eRF3与参与各种细胞代谢过程的蛋白质的相互作用确保了这种连接。如mRNA从细胞核转运到细胞质(Dbp5/DDX19和Gle1),核糖体再循环(Rli1/ABCE1),mRNA降解(Upf蛋白),和翻译启动(Pab1/PABP)。除了基因控制,翻译终止有表观遗传控制。这种机制与Sup35蛋白的朊病毒聚合形成[PSI^(+)]朊病毒有关。维持[PSI^(+)]朊病毒,像其他酵母病毒一样,需要分子伴侣和蛋白质分选因子系统的操作。该综述详细考虑了翻译终止因子与参与各种细胞过程的蛋白质的相互作用。