Abstract：
The increasing emergence of bacterial resistance is a challenge for the research community, thus novel antibacterial agents should be developed. Metal nanoparticles are promising antibacterial agents and could solve the problem of antibiotic resistance. Herein, we used Gardenia thailandica methanol extract (GTME) to biogenically synthesise zinc oxide nanoparticles (ZnO-NPs). The characterisation of ZnO-NPs was performed by UV spectroscopy, FTIR, scanning and transmission electron microscopes, dynamic light scattering, and X-ray diffraction. The antibacterial activity of ZnO-NPs was studied both in vitro and in vivo against Pseudomonas aeruginosa clinical isolates. Its minimum inhibitory concentration values ranged from 2 to 64 µg/mL, and it significantly decreased the membrane integrity and resulted in a significant increase in the inner and outer membrane permeability. Also, the ZnO-NPs treated cells possessed a distorted and deformed shape when examined by scanning electron microscope. The in vivo study (biochemical parameters and histological investigation) was conducted and it revealed a protective effect of ZnO-NPs against the deleterious influences of P. aeruginosa bacteria on lung, liver, and kidney tissues. LC-ESI-MS/MS revealed a phytochemical tentative identification of 57 compounds for the first time. We propose that GTME is a useful source for ZnO-NPs which has a promising antibacterial activity.
摘要：
细菌耐药性的日益增加对研究界来说是一个挑战，因此应该开发新的抗菌剂。金属纳米粒子是有前途的抗菌剂，可以解决抗生素耐药性问题。在这里，我们使用栀子甲醇提取物（GTME）生物合成氧化锌纳米颗粒（ZnO-NP）。ZnO-NP的表征是通过紫外光谱进行的，FTIR,扫描和透射电子显微镜，动态光散射,和X射线衍射。在体外和体内研究了ZnO-NP对铜绿假单胞菌临床分离株的抗菌活性。其最小抑制浓度值范围为2至64µg/mL，并且它显着降低了膜的完整性，并导致内外膜渗透性显着增加。此外，通过扫描电子显微镜检查，ZnO-NP处理的电池具有扭曲和变形的形状。进行了体内研究（生化参数和组织学研究），它揭示了ZnO-NP对铜绿假单胞菌对肺的有害影响的保护作用。肝脏,和肾脏组织。LC-ESI-MS/MS首次揭示了57个化合物的植物化学初步鉴定。我们认为GTME是ZnO-NP的有用来源，具有很好的抗菌活性。
