Fecal samples collected in France between 2011 and 2016 from patients with normal colonoscopy (n = 25), adenoma (n = 23), or colorectal cancer at different stages (n = 81) were tested by PCR for the presence of SGG, B. fragilis, F. nucleatum, P. micra, and the pks island. Relative quantification of SGG, F. nucleatum, and P. micra in stools was performed by qPCR.
SGG prevalence was significantly increased in the CRC group. Our results also revealed i) a strong and significant increase of toxinogenic B. fragilis in patients with early-stage adenoma and of pks island at late-stage CRC and ii) increased levels of F. nucleatum and P. micra in the stools of CRC patients. Furthermore, the simultaneous detection of these five bacterial markers was only found in CRC patients.
Our results indicate that the prevalence or relative levels of CRC-associated bacteria vary during CRC development. Among them, B. fragilis (bft+) was singled out as the sole pathobiont detected at the early adenoma stage.
2011年至2016年在法国从结肠镜检查正常的患者(n=25)收集的粪便样本,腺瘤(n=23),或结直肠癌在不同阶段(n=81)通过PCR检测SGG的存在,B.脆弱,F.核仁,P.micra,还有Pks岛.SGG的相对量化,F.核仁,通过qPCR进行粪便中的P。
在CRC组中SGG患病率显著增加。我们的结果还表明,i)早期腺瘤患者的致毒脆弱芽孢杆菌和晚期CRC患者的pks岛的强烈且显着增加,以及ii)粪便中的F.nucleatum和P.micra水平升高CRC患者。此外,仅在CRC患者中同时检测到这5种细菌标志物.
我们的结果表明,CRC相关细菌的患病率或相对水平在CRC发展过程中有所不同。其中,将脆弱芽孢杆菌(bft)作为在腺瘤早期阶段检测到的唯一病原体。