PDF(Pubmed)
Abstract：
UNASSIGNED: Midstream urine specimen cultures play an important role in assisting clinicians to choose antibiotics to remove urinary tract pathogens. At present, there are many culture methods for obtaining midstream urine specimens. In this article, different urine culture methods were compared to improve the detection rate of pathogenic bacteria in patients with urinary tract infections (UTIs).
UNASSIGNED: Urina sanguinis specimens were collected from 90 outpatients and inpatients who had UTI symptoms, had been clinically diagnosed with UTI between February and March 2021, and had been tested within 2 h using the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method. After incubation at 35 °C with 5% carbon dioxide (CO2) for 48 h, the detection rates of pathogenic bacteria in UTIs were compared among the three methods.
UNASSIGNED: A total of 75 strains of pathogenic bacteria were detected in the 90 specimens. The positive detection rates of pathogenic microorganisms were 23.33%, 73.33%, and 75.56% for the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method, respectively. Among the patients who used antibiotics before the collection of the urine specimens, the positive detection rates of pathogenic microorganism were 16.00%, 66.00% and 66.00% for the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method, respectively. In the specimens grown aseptically using the conventional 1-µL culture method, a similar average number of colonies was found using the modified 100-µL culture method and the centrifugal urine sediment culture method. Among the specimens with bacterial growth in the conventional 1-µL culture method, the average colony numbers of the three methods were similar.
UNASSIGNED: The modified 100-µL culture method and the centrifugal urine sediment culture method greatly improved the positive detection rates of pathogenic bacteria in patients with UTIs.
UNASSIGNED: Urina sanguinis specimens were collected from 90 outpatients and inpatients who had UTI symptoms, had been clinically diagnosed with UTI between February and March 2021, and had been tested within 2 h using the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method. After incubation at 35 °C with 5% carbon dioxide (CO2) for 48 h, the detection rates of pathogenic bacteria in UTIs were compared among the three methods.
UNASSIGNED: A total of 75 strains of pathogenic bacteria were detected in the 90 specimens. The positive detection rates of pathogenic microorganisms were 23.33%, 73.33%, and 75.56% for the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method, respectively. Among the patients who used antibiotics before the collection of the urine specimens, the positive detection rates of pathogenic microorganism were 16.00%, 66.00% and 66.00% for the conventional 1-µL culture method, the modified 100-µL culture method, and the centrifugal urine sediment culture method, respectively. In the specimens grown aseptically using the conventional 1-µL culture method, a similar average number of colonies was found using the modified 100-µL culture method and the centrifugal urine sediment culture method. Among the specimens with bacterial growth in the conventional 1-µL culture method, the average colony numbers of the three methods were similar.
UNASSIGNED: The modified 100-µL culture method and the centrifugal urine sediment culture method greatly improved the positive detection rates of pathogenic bacteria in patients with UTIs.
摘要：
UASSIGNED：中游尿液标本培养在协助临床医生选择抗生素以去除尿路病原体方面起着重要作用。目前,获得中游尿液标本的培养方法很多。在这篇文章中,比较不同尿培养方法,提高尿路感染(UTIs)患者病原菌检出率。
UnASSIGNED：从90名有UTI症状的门诊患者和住院患者中收集血尿标本，在2021年2月至3月期间被临床诊断为UTI，并在2小时内使用常规1μL培养方法进行了测试，改良的100微升培养方法，和离心尿沉渣培养方法。在35°C与5%二氧化碳（CO2）孵育48小时后，比较3种方法对UTI病原菌的检出率。
UNASSIGNED:在90份标本中共检测到75株病原菌。病原微生物阳性检出率为23.33%,73.33%,常规1μL培养方法为75.56%，改良的100微升培养方法，和离心尿沉渣培养方法，分别。在收集尿液样本之前使用抗生素的患者中，病原微生物阳性检出率为16.00%,常规1μL培养方法为66.00%和66.00%，改良的100微升培养方法，和离心尿沉渣培养方法，分别。在使用常规1微升培养方法无菌生长的标本中，使用改良的100微升培养方法和离心尿沉渣培养方法发现了相似的平均菌落数。在常规1μL培养方法中细菌生长的标本中，三种方法的平均菌落数相似。
UNASSIGNED：改良的100-µL培养方法和离心尿沉渣培养方法大大提高了UTIs患者病原菌的阳性检出率。
UnASSIGNED：从90名有UTI症状的门诊患者和住院患者中收集血尿标本，在2021年2月至3月期间被临床诊断为UTI，并在2小时内使用常规1μL培养方法进行了测试，改良的100微升培养方法，和离心尿沉渣培养方法。在35°C与5%二氧化碳（CO2）孵育48小时后，比较3种方法对UTI病原菌的检出率。
UNASSIGNED:在90份标本中共检测到75株病原菌。病原微生物阳性检出率为23.33%,73.33%,常规1μL培养方法为75.56%，改良的100微升培养方法，和离心尿沉渣培养方法，分别。在收集尿液样本之前使用抗生素的患者中，病原微生物阳性检出率为16.00%,常规1μL培养方法为66.00%和66.00%，改良的100微升培养方法，和离心尿沉渣培养方法，分别。在使用常规1微升培养方法无菌生长的标本中，使用改良的100微升培养方法和离心尿沉渣培养方法发现了相似的平均菌落数。在常规1μL培养方法中细菌生长的标本中，三种方法的平均菌落数相似。
UNASSIGNED：改良的100-µL培养方法和离心尿沉渣培养方法大大提高了UTIs患者病原菌的阳性检出率。
