背景:油会通过炎症损害移植结果,这导致了体积保留率低至20%的不可预测性。现有的除油技术相对低效。在这项研究中,采用一种新的方法,通过絮凝和离心去除油,制备浓缩脱油脂肪(CDF)。这项研究中提出的假设是CDF将通过提高纯化效率和减少炎症而表现出改善的体积保留和质量。
方法:这项基础研究涉及使用从接受腹部吸脂术的女性获得的样本进行的体外和体内实验。通过絮凝和离心制备CDF。在体外实验中,采用钙黄绿素乙酰氧基甲酯(AM)染色的活细胞和碘化丙啶(PI)染色的死核评估了两组的脂肪微观结构:科尔曼脂肪组和CDF组.此外,使用葡萄糖转运试验(GTT)评估两组的葡萄糖摄取能力.在体内实验中,该研究包括三组:两个实验组(低体积浓缩脱油脂肪,LCDF;高容量浓缩脱油脂肪,HCDF)和一个对照组(科尔曼脂肪),每组10只健康雌性BALB/c裸鼠,每只小鼠皮下注射1ml移植物。8周后,收获脂肪移植物并进行体积评估,HE染色和免疫染色用于评估移植物结果。
结果:在体外实验中,CDF的浓度率为Coleman脂肪的79.6%,与15.1%以上的油分离。细胞活力,通过AM/PI染色评估,两个移植物之间没有显着差异,但是GTT的结果表明CDF的组织活力高于Coleman脂肪。在体内实验中,CDF的体积保留率高于Coleman脂肪,以水驱替来衡量。组织病理学评分显示HCDF组和LCDF组脂肪结构完整,空泡少,炎症,与科尔曼脂肪相比,纤维化。此外,LCDF组和HCDF组的perilipin阳性面积百分比高于Coleman组,表明使用浓缩脱油脂肪改善了移植物质量和结果。
结论:“浓缩脱油脂肪”是指通过絮凝和离心去除油的自体脂肪移植物。该过程增加体积保留和活细胞并减少浸润的炎性细胞。
方法:本期刊要求作者为每篇文章分配一定程度的证据。对于这些循证医学评级的完整描述,请参阅目录或在线作者说明www。springer.com/00266.
BACKGROUND: Oil compromises graft outcomes via inflammation, which accounts for the unpredictability of volume retention rates as low as 20%. Existing techniques for oil removal are relatively inefficient. In this study, a novel approach was taken to prepare concentrated deoiled fat (CDF) by utilizing flocculation and
centrifugation to remove the oil. The hypothesis put forward in this study was that CDF would exhibit improved volume retention and quality by enhancing purification efficiency and reducing inflammation.
METHODS: This basic research involved both in vitro and in vivo experiments using samples obtained from women who underwent abdominal liposuction. The CDF was prepared by flocculation and
centrifugation. In the vitro experiments, the microstructure of fat was assessed using Calcein acetoxymethyl ester (AM) staining for living cells and propidium iodide (PI) staining for dead nuclei in two groups: Coleman fat group and CDF group. Additionally, the glucose uptake capacity of these two groups was evaluated using the glucose transport test (GTT). In the vivo experiments, the study included three groups: two experimental groups (low-volume concentrated deoiled fat, LCDF; high-volume concentrated deoiled fat, HCDF) and one control group (Coleman fat), with 10 healthy female BALB/c nude mice in each group, 1ml of the graft was injected subcutaneously to each mouse. After 8 weeks, the fat grafts were harvested and subjected to volume evaluation, HE staining and immunostaining for perilipin to assess graft outcomes.
RESULTS: In the vitro experiments, the concentration rate of the CDF was found to be 79.6% that of Coleman fat, with 15.1% more oil separated. Cell viability, as assessed by AM/PI staining, did not show a significant difference between the two grafts, but the results of the GTT showed that the tissue viability of the CDF was higher than that of Coleman fat. In the vivo experiments, the CDF had higher volume retention than Coleman fat, as measured by water displacement. Histopathologic scoring indicated that HCDF group and LCDF group had a more intact fat structure with fewer vacuoles, inflammation, and fibrosis compared to Coleman fat. Additionally, the percentages of perilipin-positive area in the LCDF group and HCDF group were higher than in the Coleman group, indicating improved graft quality and outcome with the use of concentrated deoiled fat.
CONCLUSIONS: \"Concentrated deoiled fat\" refers to an autologous fat graft from which oil has been removed by flocculation and
centrifugation. This process increases volume retention and viable cells and decreases infiltrated inflammatory cells.
METHODS: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .