关键词: L. plantarum Dad-13 Short-Chain Fatty Acid gummy probiotic gut microbiota modulation moderate undernutrition

Mesh : Gastrointestinal Microbiome / genetics Humans Infant Lactobacillus plantarum Malnutrition / prevention & control Probiotics RNA, Ribosomal, 16S / genetics

来  源:   DOI:10.3390/nu14051049

Abstract:
Undernutrition is associated with gut microbiota unbalance, and probiotics are believed to restore it and improve gut integrity. A randomized double-blind controlled trial was conducted to evaluate the efficacy of gummy L. plantarum Dad-13 (108-9 CFU/3 g) to prevent the progression of severe undernutrition. Two groups of moderate undernutrition infants were involved in this study, namely the placebo (n = 15) and probiotics (n = 15) groups, and were required to consume the product for 50 days. 16S rRNA sequencing and qPCR were used for gut microbiota analysis, and gas chromatography was used to analyze Short-Chain Fatty Acid (SCFA). The daily food intake of both groups was recorded using food records. Our results revealed that the probiotic group had better improvements regarding the anthropometry and nutritional status. In addition, L. plantarum Dad-13 modulated the butyric acid-producing bacteria to increase and inhibit the growth of Enterobacteriaceae. This gut modulation was associated with the increment in SCFA, especially total SCFA, propionic, and butyric acid. The number of L. plantarum was increased after the probiotic intervention. However, L. plantarum Dad-13 was not able to change the alpha and beta diversity. Therefore, L. plantarum Dad-13 has been proven to promote the growth of beneficial bacteria.
摘要:
营养不良与肠道微生物群失衡有关,和益生菌被认为可以恢复它并改善肠道完整性。进行了一项随机双盲对照试验,以评估植物乳杆菌Dad-13(108-9CFU/3g)预防严重营养不良进展的功效。两组中度营养不足的婴儿参与了这项研究,即安慰剂组(n=15)和益生菌组(n=15),并被要求消耗该产品50天。16SrRNA测序和qPCR用于肠道菌群分析,用气相色谱法分析短链脂肪酸(SCFA)。使用食物记录记录两组的每日食物摄入量。我们的结果表明,益生菌组在人体测量学和营养状况方面有更好的改善。此外,植物乳杆菌Dad-13调节产生丁酸的细菌以增加和抑制肠杆菌科的生长。这种肠道调节与SCFA的增加有关,尤其是总SCFA,丙酸,还有丁酸.益生菌干预后,植物乳杆菌的数量增加。然而,植物乳杆菌Dad-13不能改变α和β的多样性。因此,植物乳杆菌Dad-13已被证明可促进有益细菌的生长。
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