Abstract：
<b>Background and Objective:</b> <i>HIF-1α</i> and <i>EGFL7</i> are genes found in the placenta that play an important role in the regulation of trophoblast differentiation, hypoxia is glycolysis, red blood cell production and angiogenesis. Indonesia has antioxidant plants such as andaliman (<i>Zanthoxylum acanthopodium</i>) and haramonting (<i>Rhodomyrtus tomentosa</i>). This study aimed to analyze the role of <i>EGFL7</i> and <i>HIF-1α</i> genes on human trophoblast after administration of these 2 herbs. <b>Materials and Methods:</b> This study used HTR8 trophoblast cells with 4 incubation times, namely 30 min 1, 3 and 16 hrs (overnight) with a total of 48 weeks and then observed the cells. Cells were cultured in RPMI1640, then RNA isolation was performed, mRNA was reverse transcribed and analyzed using RT-PCR. <b>Results:</b> Nanoherbal <i>Zanthoxylum acanthopodium</i> (NZA) to the <i>EGFL7</i> gene, the longer the incubation time of human trophoblast cells, the less expression of the <i>EGFL7</i> gene (p<0.05). On the other hand, in the administration of Nanoherbal <i>Rhodomyrtus tomentose</i> (NRT), the longer the incubation time of human trophoblast cells, the higher the expression of the <i>EGFL7</i> gene. In the <i>HIF-1α</i> gene, only incubation time >16 hrs of human trophoblast cells treated with Nanoherbal <i>Zanthoxylum acanthopodium</i> (NZA) can reduce <i>HIF-1α</i> gene expression. However, the longer the incubation time of human trophoblast cells on the administration of Nanoherbal <i>Rhodomyrtus tomentosa</i> (NRT), the more the <i>HIF-1α</i> gene expression decreased (p<0.01). <b>Conclusion:</b> <i>Rhodomyrtus tomentosa</i> gave a more significant effect than <i>Zanthoxylum acanthopodium</i>.
摘要：
<b>背景和目的:</b><i>HIF-1α</i>和<i>EGFL7</i>是胎盘中发现的基因,在调节滋养细胞分化中起重要作用,缺氧是糖酵解，红细胞生成和血管生成。印度尼西亚有抗氧化植物，如安达里曼(<i>花椒</i>)和haramonting(<i>Rhodomyrtustomentosa</i>)。本研究旨在分析服用这两种草药后，<i>EGFL7</i>和<i>HIF-1α</i>基因在人类滋养层中的作用。<b>材料与方法:</b>本研究使用HTR8滋养层细胞进行4次孵育,即30分钟1、3和16小时（过夜），共48周，然后观察细胞。在RPMI1640中培养细胞，然后进行RNA分离，使用RT-PCR对mRNA进行逆转录和分析。<b>结果:</b>纳米草药<i>花椒</i>(NZA)到<i>EGFL7</i>基因,人类滋养层细胞的培养时间越长，<i>EGFL7</i>基因表达较少(p<0.05)。另一方面,在纳米草药的管理中<i>Rhodomyrtustomentose</i>（NRT），人类滋养层细胞的培养时间越长，<i>EGFL7</i>基因的表达越高。在<i>HIF-1α</i>基因中,用纳米草药<i>花椒</i>(NZA)处理的人滋养层细胞的培养时间>16小时才能降低<i>HIF-1α</i>基因表达。然而,给药纳米草药<i>Rhodomyrtustomentosa</i>（NRT）时，人滋养层细胞的孵育时间越长，<i>HIF-1α</i>基因表达越下降(p<0.01)。<b>结论:</b><i>与<i>花椒</i>相比,效果更显著。
