这项工作介绍了从Campomanesiaadamantium(Cambess。)O.Berg(Myrtaceae),一种在巴西发现的本地物种,俗称“guabiroba-do-campo”,在巴西塞拉多分布丰富。它因其抗风湿而被广泛使用,抗炎,止泻药,降低血液胆固醇,尿防腐剂,和净化属性。这些真菌是生活在高等植物内部的微生物,至少在它们的生命周期中,占据植物组织如叶和茎的细胞间隙。这些真菌对寄主植物无害,它们的次级代谢产物促进保护,调节生长,对抗细菌,病毒,和真菌,并促进对非生物胁迫的抗性,以及杀虫效果。使用培养基分离技术分离了与c.adamantium叶片相关的内生真菌。成长之后,根据形态类型将真菌分为几组。提取真菌基因组DNA,并进行聚合酶链反应(PCR)以扩增rRNA的ITS1-5.8S-ITS2区域,并将获得的核苷酸序列与GenBank数据库中可用的核苷酸序列进行比较,以对分离株进行分子鉴定。使用MEGA11软件构建系统发育树。结果显示子囊门的代表,并且有可能在属水平上鉴定出炭疽病属的18种真菌,Diaporthe,Epicoccum,和Neofusicoccum。
This work presents the isolation of endophytic fungi from the leaves of Campomanesia adamantium (Cambess.) O. Berg (
Myrtaceae), a native species found in Brazil and popularly known as \"guabiroba-do-campo\", with abundant distribution in the Brazilian Cerrado. It has been popularly used for its anti-rheumatic, anti-inflammatory, antidiarrheal, blood cholesterol-reducing, urinary antiseptic, and depurative properties. Theese fungi are microorganisms that live inside higher plants, at least for a period of their life cycle, occupying the intercellular spaces of plant tissues such as leaves and stems. These fungi are harmless to the host plant, and their secondary metabolites promote protection, regulate growth, combat bacteria, viruses, and fungi, and promote resistance to abiotic stress, as well as insecticidal effects. Endophytic fungi associated with the leaves of C. adamantium were isolated using the culture medium isolation technique. After growth, the fungi were divided into groups based on morphotypes. Fungal genomic DNA was extracted, and a polymerase chain reaction (PCR) was conducted to amplify the ITS1-5.8S-ITS2 regions of rRNA, and the nucleotide sequences obtained were compared with those available in the GenBank database for molecular identification of the isolates. The phylogenetic tree was constructed using MEGA 11 software. The results showed representatives of the Ascomycota phylum, and it was possible to identify at the genus level 18 fungi of the genera Colletotrichum, Diaporthe, Epicoccum, and Neofusicoccum.