Human pre-mRNA 3' end processing: reconstituting is believing.

人类前 mRNA 3 '末端加工 ： 重构是相信的。

• 影响指数: 12.89
• 发表时间: 02 2022 1
• 来源期刊: Genes Dev
• DOI: 10.1101/gad.349453.122
• PMID: 35193945
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 生物
• 作者列表: Yoon Y,Shi Y

关键词: 3′ processing; CPSF; RBBP6; RNA; RNA cleavage; RNA processing; endonuclease; gene expression; poly(A) polymerase; polyadenylation

Mesh: Humans; RNA Precursors / metabolism; RNA Processing, Post-Transcriptional; mRNA Cleavage and Polyadenylation Factors / genetics

Abstract

It is every biochemist\'s dream to reconstitute a biological process in vitro using defined components, because doing so not only reduces a biological phenomenon to one or a series of biochemical reactions, but also defines the minimal list of essential components. In this issue of Genes & Development, Boreikaite and colleagues (pp. 210-224) and Schmidt and colleagues (pp. 195-209) report their independent reconstitution of human pre-mRNA 3\' end processing.

摘要

它是每一个生物化学家的梦想，在体外使用定义的组件重建生物过程，因为这样做不仅将一种生物现象减少到一种或一系列的生化反应，但也定义了基本组件的最小列表。在本期《基因与发育》中,Boreikaite及其同事(pp.210-224)和施密特及其同事(pp.195-209)报告了他们对人类前mRNA3'末端加工的独立重建。