Abstract：
The protein adsorption onto poly(acrylic acid)-block-polystyrene (PAA22-b-PS144) polymersomes has been investigated with regard to structural features, thermodynamic aspects and biological consequences. The light scattering measurements revealed the formation of protein coronas enveloping the polymeric capsules regardless of the chemical nature of the biomacromolecules. The experiments were conducted by using lysozyme, immunoglobulin G - IgG and bovine serum albumin - BSA as model proteins due to their differences concerning size and residual surface charge at physiological pH. The protein adsorption was further confirmed by isothermal titration calorimetry, and the experimental data suggest that the phenomenon is mainly governed by hydrogen bonding and van der Waals interactions. The pre-existing protein layer via the pre-incubation in protein environments notably attenuates the cytotoxicity of the nanomaterial compared to the pristine counterparts. This approach can possibly be extended to different types of assemblies when intermolecular interactions are able to induce protein adsorption and the development of protein coronas around nanoparticles. Such fairly simple method may be convenient to engineer safer nanomaterials towards a variety of biomedical applications when the nanotoxicity is an issue. Additionally, the strategy can possibly be used to tailor the surface properties of nanoparticles by adsorbing specific proteins for targeting purposes.
摘要：
已经研究了蛋白质在聚（丙烯酸）-嵌段聚苯乙烯（PAA22-b-PS144）聚合物上的吸附，热力学方面和生物学后果。光散射测量表明，无论生物大分子的化学性质如何，都会形成包裹聚合物胶囊的蛋白质电晕。实验采用溶菌酶,免疫球蛋白G-IgG和牛血清白蛋白-BSA作为模型蛋白，因为它们在生理pH下的大小和残留表面电荷方面存在差异。通过等温滴定量热法进一步证实了蛋白质的吸附，实验数据表明，该现象主要受氢键和范德华相互作用控制。与原始对应物相比,通过在蛋白质环境中预孵育的预先存在的蛋白质层显著减弱了纳米材料的细胞毒性。当分子间相互作用能够诱导蛋白质吸附和纳米颗粒周围蛋白质电晕的发展时，这种方法可能会扩展到不同类型的组件。当纳米毒性是一个问题时，这种相当简单的方法可以方便地将更安全的纳米材料设计为各种生物医学应用。此外,该策略可能用于通过吸附特定蛋白质以靶向目的来定制纳米颗粒的表面性质。
