Abstract：
Autologous lipotransfer aims to restore aging-associated volume loss, but with low predictability owing to 20-90% first-year loss of transferred fat. Enrichment by adipose-derived stem cells within the stromal vascular fraction (SVF) aims to improve volume retention through their differentiation potential and paracrine actions exerted by secreted trophic and angiogenic factors. Assessing studies lacked split-face designs, and used multitudes of enrichment ratios, preparation techniques and evaluation methods ending in contradictory reports regarding enrichment advantage.
To test whether enriching the autologous fat graft with SVF will increase its residual volume as compared to non-enriched graft. A standardized enrichment protocol and ratio and objective assessment were employed.
In a split-face design, and after random assignment, bilateral temple augmentation using non-enriched versus SVF-enriched autologous lipotransfer were compared in middle-aged females otherwise healthy non-pregnant or breast-feeding females abstaining from esthetic or weight-controlling procedures. Temple volume scale (TVS), skin layers\' thickness measured by ultrasound biomicroscopy (UBM), visual analog scale for patients\' satisfaction, and side effects were blindly assessed at 1 week, 3 months, and 6 months.
In the included 15 females, TVS was significantly lower (0.5 ± 0.5 versus 1.1 ± 0.7, P = 0.0001), and% hypodermal augmentation was significantly higher (70.92 ± 58.09 versus 18.93 ± 19.33, P = 0.001) on the SVF-enriched side at 6 months. Patient satisfaction was similar bilaterally (P = 1), as were sequelae frequencies as lumping, edema, and ecchymosis.
SVF enrichment of transferred fat significantly improved its residual volume at 6 months; a conclusion that needs further validation. UBM was an informative objective tool for the following temple skin thickness changes. Trial registration clinical trials.gov (NCT03965936).
To test whether enriching the autologous fat graft with SVF will increase its residual volume as compared to non-enriched graft. A standardized enrichment protocol and ratio and objective assessment were employed.
In a split-face design, and after random assignment, bilateral temple augmentation using non-enriched versus SVF-enriched autologous lipotransfer were compared in middle-aged females otherwise healthy non-pregnant or breast-feeding females abstaining from esthetic or weight-controlling procedures. Temple volume scale (TVS), skin layers\' thickness measured by ultrasound biomicroscopy (UBM), visual analog scale for patients\' satisfaction, and side effects were blindly assessed at 1 week, 3 months, and 6 months.
In the included 15 females, TVS was significantly lower (0.5 ± 0.5 versus 1.1 ± 0.7, P = 0.0001), and% hypodermal augmentation was significantly higher (70.92 ± 58.09 versus 18.93 ± 19.33, P = 0.001) on the SVF-enriched side at 6 months. Patient satisfaction was similar bilaterally (P = 1), as were sequelae frequencies as lumping, edema, and ecchymosis.
SVF enrichment of transferred fat significantly improved its residual volume at 6 months; a conclusion that needs further validation. UBM was an informative objective tool for the following temple skin thickness changes. Trial registration clinical trials.gov (NCT03965936).
摘要：
自体脂肪转移旨在恢复与衰老相关的体积损失，但由于第一年减少20-90％的转移脂肪，可预测性较低。脂肪来源的干细胞在基质血管部分（SVF）中的富集旨在通过其分化潜力和分泌的营养和血管生成因子发挥的旁分泌作用来改善体积保留。评估研究缺乏分面设计，并使用了多种富集率，制备技术和评价方法以关于富集优势的矛盾报道结尾。
测试与未富集的移植物相比,用SVF富集自体脂肪移植物是否会增加其残余体积。采用标准化的富集方案和比例以及客观评估。
在分面设计中，在随机分配之后，比较了使用非富含与富含SVF的自体脂肪转移的双侧太阳穴增大术在中年女性中进行的比较,这些中年女性或健康的非妊娠或母乳喂养女性放弃了美学或体重控制程序.寺庙体积量表（TVS），通过超声生物显微镜（UBM）测量的皮肤层厚度，患者满意度的视觉模拟量表，副作用在1周时盲目评估，3个月,和6个月。
在15名女性中，TVS显著降低(0.5±0.5vs1.1±0.7,P=0.0001),在6个月时，富含SVF的一侧的皮下增强率明显更高（70.92±58.09比18.93±19.33，P=0.001）。患者满意度相似（P=1），后遗症的频率也是如此，水肿,还有瘀斑.
转移脂肪的SVF富集显著改善了其在6个月时的残余体积;该结论需要进一步验证。UBM是以下太阳穴皮肤厚度变化的信息客观工具。临床试验注册(NCT03965936)。
测试与未富集的移植物相比,用SVF富集自体脂肪移植物是否会增加其残余体积。采用标准化的富集方案和比例以及客观评估。
在分面设计中，在随机分配之后，比较了使用非富含与富含SVF的自体脂肪转移的双侧太阳穴增大术在中年女性中进行的比较,这些中年女性或健康的非妊娠或母乳喂养女性放弃了美学或体重控制程序.寺庙体积量表（TVS），通过超声生物显微镜（UBM）测量的皮肤层厚度，患者满意度的视觉模拟量表，副作用在1周时盲目评估，3个月,和6个月。
在15名女性中，TVS显著降低(0.5±0.5vs1.1±0.7,P=0.0001),在6个月时，富含SVF的一侧的皮下增强率明显更高（70.92±58.09比18.93±19.33，P=0.001）。患者满意度相似（P=1），后遗症的频率也是如此，水肿,还有瘀斑.
转移脂肪的SVF富集显著改善了其在6个月时的残余体积;该结论需要进一步验证。UBM是以下太阳穴皮肤厚度变化的信息客观工具。临床试验注册(NCT03965936)。
