Abstract：
BACKGROUND: Fibrous dysplasia (FD) and cemento-ossifying fibroma (COF) are the most common gnathic fibro-osseous lesions. These diseases exhibit remarkable overlap of several clinicopathological aspects, and differential diagnosis depends on the combination of histopathological, radiographic, and clinical aspects. Their molecular landscape remains poorly characterized, and herein, we assessed their proteomic and phosphoproteomic profiles.
METHODS: The quantitative differences in protein profile of FD and COF were assessed by proteomic and phosphoproteomic analyses of formalin-fixed paraffin-embedded tissue samples. Pathway enrichment analyses with differentially regulated proteins were performed.
RESULTS: FD and COF exhibited differential regulation of pathways related to extracellular matrix organization, cell adhesion, and platelet and erythrocytes activities. Additionally, these lesions demonstrated distinct abundance of proteins involved in osteoblastic differentiation and tumorigenesis and differential abundance of phosphorylation of Ser61 of Yes-associated protein 1 (YAP1).
CONCLUSIONS: In summary, despite the morphological similarity between these diseases, our results demonstrated that COF and DF present numerous quantitative differences in their proteomic profiles. These findings suggest that these fibro-osseous lesions trigger distinct molecular mechanisms during their pathogenesis. Moreover, some proteins identified in our analysis could serve as potential biomarkers for differential diagnosis of these diseases after further validation.
METHODS: The quantitative differences in protein profile of FD and COF were assessed by proteomic and phosphoproteomic analyses of formalin-fixed paraffin-embedded tissue samples. Pathway enrichment analyses with differentially regulated proteins were performed.
RESULTS: FD and COF exhibited differential regulation of pathways related to extracellular matrix organization, cell adhesion, and platelet and erythrocytes activities. Additionally, these lesions demonstrated distinct abundance of proteins involved in osteoblastic differentiation and tumorigenesis and differential abundance of phosphorylation of Ser61 of Yes-associated protein 1 (YAP1).
CONCLUSIONS: In summary, despite the morphological similarity between these diseases, our results demonstrated that COF and DF present numerous quantitative differences in their proteomic profiles. These findings suggest that these fibro-osseous lesions trigger distinct molecular mechanisms during their pathogenesis. Moreover, some proteins identified in our analysis could serve as potential biomarkers for differential diagnosis of these diseases after further validation.
摘要：
背景：纤维发育不良（FD）和骨水泥骨化性纤维瘤（COF）是最常见的颌骨纤维骨性病变。这些疾病在几个临床病理方面表现出明显的重叠，鉴别诊断取决于组织病理学的结合，射线照相,和临床方面。他们的分子景观特征仍然很差，在这里，我们评估了他们的蛋白质组和磷酸化蛋白质组。
方法：通过福尔马林固定石蜡包埋组织样品的蛋白质组学和磷酸化蛋白质组学分析评估FD和COF的蛋白质谱的定量差异。用差异调节的蛋白质进行通路富集分析。
结果：FD和COF表现出与细胞外基质组织相关的通路的差异调节，细胞粘附，血小板和红细胞活性。此外,这些病变显示了与成骨细胞分化和肿瘤发生有关的蛋白质的丰度和Yes相关蛋白1(YAP1)的Ser61磷酸化的丰度差异.
结论：总之，尽管这些疾病在形态上相似,我们的结果表明，COF和DF在其蛋白质组学谱中存在许多数量差异。这些发现表明，这些纤维骨病变在其发病机理中触发了不同的分子机制。此外，我们分析中发现的一些蛋白质在进一步验证后可作为这些疾病鉴别诊断的潜在生物标志物.
方法：通过福尔马林固定石蜡包埋组织样品的蛋白质组学和磷酸化蛋白质组学分析评估FD和COF的蛋白质谱的定量差异。用差异调节的蛋白质进行通路富集分析。
结果：FD和COF表现出与细胞外基质组织相关的通路的差异调节，细胞粘附，血小板和红细胞活性。此外,这些病变显示了与成骨细胞分化和肿瘤发生有关的蛋白质的丰度和Yes相关蛋白1(YAP1)的Ser61磷酸化的丰度差异.
结论：总之，尽管这些疾病在形态上相似,我们的结果表明，COF和DF在其蛋白质组学谱中存在许多数量差异。这些发现表明，这些纤维骨病变在其发病机理中触发了不同的分子机制。此外，我们分析中发现的一些蛋白质在进一步验证后可作为这些疾病鉴别诊断的潜在生物标志物.
