Abstract：
In current work, with elaborate designs of G-quadruplex containing \"Y\" junction structures, we demonstrate the construction of several new and label-free electrochemical logic gate operations (OR, AND, NOR, and NAND) by defining two distinct biomolecules, human 8-oxo-7,8-dihydroguanine DNA glycosylase 1 (hOGG1) and microRNA-141 (miRNA-141), as the inputs. The \"Y\" junction structures are immobilized onto the surface of gold electrode as the signal transduction platform. The presence of the input molecules with different combinations can alter the \"Y\" junction structures to disrupt the formation of the complete G-quadruplexes via 8-oxoG-site specific cleavage and miRNA-141-triggered displacement of the \"Y\" junctions. Subsequent association of hemin with the G-quadruplex sequences thus yields significant current variation outputs upon electrochemical reduction of hemin on the electrode, leading to the successful function of different logic operations without the involvement of labeling the DNA sequences with electro-active species. Featured with the advantages of multiple logic operations with distinct inputs and the label-free electrochemical format, such molecular logic gates can potentially provide promising opportunities for the development of simple and robust biological logic gates for various applications.
摘要：
在目前的工作中,精心设计了包含“Y”结结构的G-四链体，我们演示了几个新的和无标签的电化学逻辑门操作(OR，AND,NOR,和NAND)通过定义两个不同的生物分子，人8-氧代-7,8-二氢鸟嘌呤DNA糖基化酶1（hOGG1）和microRNA-141（miRNA-141），作为输入。\"Y\"连接结构固定在金电极表面作为信号转导平台。具有不同组合的输入分子的存在可以改变\“Y\”连接结构，从而通过8-oxoG位点特异性裂解和miRNA-141触发的\“Y\”置换破坏完整G-四链体的形成连接。因此，血红素与G-四链体序列的随后结合在电极上的血红素的电化学还原后产生了显着的电流变化输出。导致不同逻辑运算的成功功能，而无需用电活性物质标记DNA序列。具有具有不同输入和无标签电化学格式的多种逻辑运算的优点，这种分子逻辑门可以潜在地为开发用于各种应用的简单和强大的生物逻辑门提供有希望的机会。
