关键词: Adhesins Bacteria Bacterial lectins Biofilm Fluorescent staining Glycan microarrays Knockouts Mutants Wild type t-Test

Mesh : Acinetobacter baumannii / metabolism Humans Microarray Analysis / methods Polysaccharides / metabolism Staining and Labeling

来  源:   DOI:10.1007/978-1-0716-2148-6_14

Abstract:
The use of glycan microarrays to study carbohydrate interactions of bacterial cells is of great interest owing to the key roles these interactions play in bacterial colonization and infection of a host. In this chapter, the methods to fluorescently stain Gram-positive or Gram-negative bacteria and profiling them for glycan interactions using glycan microarrays are described in detail. The application of the Student\'s t-test to glycan microarray data using an example data set comparing glycan microarray binding of an Acinetobacter baumannii wild type and mutant strain is also described in step-by-step detail.
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