{Reference Type}: Journal Article
{Title}: Bacterial Staining and Profiling for Glycan Interactions on Glycan Microarrays for t-Test Calculation.
{Author}: Cunningham S;Gerlach JQ;Flannery A;Kilcoyne M;
{Journal}: Methods Mol Biol
{Volume}: 2460
{Issue}: 0
{Year}: 2022
暂无{DOI}: 10.1007/978-1-0716-2148-6_14
{Abstract}: The use of glycan microarrays to study carbohydrate interactions of bacterial cells is of great interest owing to the key roles these interactions play in bacterial colonization and infection of a host. In this chapter, the methods to fluorescently stain Gram-positive or Gram-negative bacteria and profiling them for glycan interactions using glycan microarrays are described in detail. The application of the Student's t-test to glycan microarray data using an example data set comparing glycan microarray binding of an Acinetobacter baumannii wild type and mutant strain is also described in step-by-step detail.