PDF(Pubmed)
Abstract：
Many chronic diseases are associated with decreased abundance of the gut commensal Faecalibacterium prausnitzii. This strict anaerobe can grow on dietary fibers, e.g., prebiotics, and produce high levels of butyrate, often associated to epithelial metabolism and health. However, little is known about other F. prausnitzii metabolites that may affect the colonic epithelium. Here, we analyzed prebiotic cross-feeding between F. prausnitzii and intestinal epithelial (Caco-2) cells in a \"Human-oxygen Bacteria-anaerobic\" coculture system. Inulin-grown F. prausnitzii enhanced Caco-2 viability and suppressed inflammation- and oxidative stress-marker expression. Inulin-grown F. prausnitzii produced excess butyrate and fructose, but only fructose efficiently promoted Caco-2 growth. Finally, fecal microbial taxonomy analysis (16S sequencing) from healthy volunteers (n = 255) showed the strongest positive correlation for F. prausnitzii abundance and stool fructose levels. We show that fructose, produced and accumulated in a fiber-rich colonic environment, supports colonic epithelium growth, while butyrate does not.
摘要：
许多慢性疾病与肠道共生假单胞菌丰度降低有关。这种严格的厌氧菌可以在膳食纤维上生长，例如，益生元,产生高水平的丁酸盐，通常与上皮代谢和健康有关。然而,对可能影响结肠上皮的其他F.prausnitzi代谢产物知之甚少。这里,我们在“人氧细菌-厌氧”共培养系统中分析了普氏F.prausnitzii和肠上皮细胞（Caco-2）之间的益生元交叉饲喂。菊粉生长的F.prausnitzii增强了Caco-2的活力，并抑制了炎症和氧化应激标记的表达。菊粉生长的F.prausnitzii产生过量的丁酸和果糖，但只有果糖才能有效促进Caco-2的生长。最后,来自健康志愿者（n=255）的粪便微生物分类学分析（16S测序）显示，普氏弧菌丰度和粪便果糖水平的正相关最强。我们证明果糖，在富含纤维的结肠环境中产生和积累，支持结肠上皮生长，而丁酸则没有。
