Abstract：
Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been focused on as a green strategy to convert CO2 into valuable products such as fatty acids. In this study, a CO2 utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO2 in the oleaginous yeast Yarrowia lipolytica, thereby increasing the production of fatty acids. In the recombinant strain in which the CO2 utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO2.
摘要：
非光合微生物的代谢工程以增加CO2的利用已被关注作为将CO2转化为有价值的产物如脂肪酸的绿色策略。在这项研究中,形成了涉及碳酸酐酶和生物素羧化酶的CO2利用途径，以在含油酵母Yarrowialipolytica中回收CO2，从而增加脂肪酸的产量。在引入CO2利用途径的重组菌株中，脂肪酸产量为10.7g/L，比野生型菌株高1.5倍。与野生型菌株相比,所得菌株的干细胞质量增加1.4倍。此外,亚油酸在最终菌株的脂肪酸组成中为47.7％，与野生型菌株相比增加了11.6%。这些结果可以作为通过直接利用CO2开发高效和环保工艺的基本技术。
