PDF(Pubmed)
Abstract：
RNA modifications play an essential role in determining RNA fate. Recent studies have revealed the effects of such modifications on all steps of RNA metabolism. These modifications range from the addition of simple groups, such as methyl groups, to the addition of highly complex structures, such as sugars. Their consequences for translation fidelity are not always well documented. Unlike the well-known m6A modification, they are thought to have direct effects on either the folding of the molecule or the ability of tRNAs to bind their codons. Here we describe how modifications found in tRNAs anticodon-loop, rRNA, and mRNA can affect translation fidelity, and how approaches based on direct manipulations of the level of RNA modification could potentially be used to modulate translation for the treatment of human genetic diseases.
摘要：
RNA修饰在决定RNA命运中起着至关重要的作用。最近的研究已经揭示了这种修饰对RNA代谢的所有步骤的影响。这些修改范围从简单组的添加，如甲基，添加高度复杂的结构，比如糖。它们对翻译保真度的影响并不总是有据可查的。与众所周知的m6A改型不同，它们被认为对分子的折叠或tRNA结合其密码子的能力有直接影响。在这里，我们描述了在tRNA反密码子环中发现的修饰，rRNA,mRNA可以影响翻译保真度，以及基于直接操作RNA修饰水平的方法如何潜在地用于调节翻译以治疗人类遗传疾病。
