Abstract：
Methods for efficient cyclodextrin-induced lipid exchange have been developed in our lab. These make it possible to almost completely replace the lipids in the outer leaflet of artificial membranes or the plasma membranes of living cells with exogenous lipids. Lipid replacement/substitution allows detailed studies of how lipid composition and asymmetry influence the structure and function of membrane domains and membrane proteins. In this review, we both summarize progress on cyclodextrin exchange in cells, mainly by the use of methyl-alpha cyclodextrin to exchange phospholipids and sphingolipids, and discuss the issues to consider when carrying out lipid exchange experiments upon cells. Issues that impact interpretation of lipid exchange are also discussed. This includes how overly naïve interpretation of how lipid exchange-induced changes in domain formation can impact protein function.
摘要：
在我们的实验室中已经开发了有效的环糊精诱导的脂质交换的方法。这些使得可以用外源脂质几乎完全替代人造膜的外小叶或活细胞的质膜中的脂质。脂质置换/置换允许详细研究脂质组成和不对称性如何影响膜结构域和膜蛋白的结构和功能。在这次审查中,我们都总结了细胞中环糊精交换的进展，主要通过使用甲基-α-环糊精来交换磷脂和鞘脂，并讨论了在细胞上进行脂质交换实验时要考虑的问题。还讨论了影响脂质交换解释的问题。这包括对脂质交换诱导的结构域形成变化如何影响蛋白质功能的过度天真解释。
